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The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This five-color system allows co-amplification and fluorescent detection of the 13 core CODIS (US) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the 12 core European Standard Set loci (TH01, vWA, FGA, D21S11, D3S1358, D8S1179, D18S51, D10S1248, D22S1045, D2S441, D1S1656 and D12S391) and Amelogenin for gender determination. In addition, the male-specific DYS391 locus is included to identify null Y ...
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The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. This five-color system allows co-amplification and fluorescent detection of the 13 core CODIS (US) loci (CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51 and D21S11), the 12 core European Standard Set loci (TH01, vWA, FGA, D21S11, D3S1358, D8S1179, D18S51, D10S1248, D22S1045, D2S441, D1S1656 and D12S391) and Amelogenin for gender determination. In addition, the male-specific DYS391 locus is included to identify null Y allele results for Amelogenin. The Penta D, Penta E, D2S1338 and D19S433 loci are included to increase discrimination and allow searching of databases that include profiles with these popular loci. This extended panel of STR markers is intended to satisfy both CODIS and ESS recommendations.
The PowerPlex® Fusion System works well with extracted DNA samples, including low amounts of template DNA, mixtures and inhibitor-laden samples. The PowerPlex® Fusion System also is compatible with direct amplification, enabling streamlined STR databasing efforts. Amplification can be successfully performed with sample types such as FTA® card punches as well as pretreated swabs, Bode Buccal DNA Collector™ punches or S&S 903 punches. Fast cycling conditions used with the PowerPlex® Fusion System reduce sample-processing time for all samples.
The PowerPlex® Fusion System is compatible with the ABI PRISM® 3100 and 3100-Avant Genetic Analyzers and Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.
Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download.
The PowerPlex® Fusion System was given NDIS approval in March 2013 for NDIS CODIS databasing.
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PowerPlex® Fusion System
2800M Control DNA
CC5 Internal Lane Standard 500
PowerPlex® Fusion 5X Primer Pair Mix
Water, Amplification Grade
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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PowerPlex® 5-Dye Matrix Standards, 3100/3130
CC5 Matrix, 5-Dye (3100/3130)
CXR-ET Matrix, 5-Dye (3100/3130)
TMR-ET Matrix, 5-Dye (3100/3130)
JOE Matrix, 5-Dye (3100/3130)
Fluorescein Matrix, 5-Dye (3100/3130)
Helix Cabinet, Freezer, Refrigerator
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.
Store kit at –20°C. Upon receipt, move 2800M Control DNA to 4°C storage.
For product intended use please see Patents & Disclaimers tab.
This system allows co-amplification of Amelogenin, D3S1358, D1S1656, D2S441, D10S1248, D13S317 and Penta E labeled with fluorescein; D16S539, D18S51, D2S1338, CSF1PO and Penta D labeled with JOE; TH01, vWA, D21S11, D7S820, D5S818, TPOX and DYS391 labeled with TMR-ET; and D8S1179, D12S391, D19S433, FGA and D22S1045 labeled with CXR-ET. The CC5 Internal Lane Standard 500 (CC5 ILS 500) is labeled with CC5 dye and contains 21 DNA fragments of 60, 65, 80, 100, 120, 140, 160, 180, 200, 225, 250, 275, 300, 325, 350, 375, 400, 425, 450, 475 and 500 bases in length.
Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection).
Amplified products were separated on an Applied Biosystems® 3130xl Genetic Analyzer (3kV, 5-second injection). Percentage of alleles detected are indicated for each system.
Following incubation, 2µl of extract was added to the PCR amplification mix and amplified as described in the PowerPlex® Fusion System Technical Manual TMD039.
DC2402, DC2408, DG4700, DD7101, DG1521, DW0991 Not For Medical Diagnostic Use.
DC2402, DC2408 U.S. Pat. No. 6,242,235, Australian Pat. No. 761757, Canadian Pat. No. 2,335,153 and other patents and patents pending.
DC2402, DC2408 U.S. Pat. No. 6,238,863 and other patents and patents pending.
DC2402, DC2408 Australian Pat. No. 724531, Canadian Pat. No. 2,251,793 and other patents and patents pending.
DC2402, DC2408, DG4700 This product or portions thereof is manufactured and sold under license from GE Healthcare under Australia Pat. No. 692230, Austria Pat. No. E236994, Belgium Pat. No. 0743987, Canada Pat. No. 2231475, EP Pat. Nos. 0743987 and 0851867, France Pat. Nos. 0743987 and 0851867, Germany Pat. Nos. 19581489, 69530286.8 and 0851867, Italy Pat. Nos. 0743987 and 0851867, Japan Pat. No. 3066984, Liechtenstein Pat. Nos. 0743987 and 0851867, Netherlands Pat. Nos. 0743987 and 0851867, Spain Pat. Nos. 2197193 and 2173310, Sweden Pat. Nos. 0743987 and 0851867, Switzerland Pat. Nos. 0743987 and 0851867, United Kingdom Pat. Nos. 0743987 and 0851867, U.S. Pat. Nos. 5,654,419, 5,688,648, 5,869,255, 6,177,247, 5,707,804, 6,028,190, 6,544,744, 7,015,000 and 5,728,528 and other pending and foreign patent applications.
End User Terms and Conditions
Acceptance. These terms and conditions shall govern the purchase, use, transfer and acceptance of the products described in the purchase order, quotation or invoice, which products are sold and distributed by Promega to the buyer/transferee of such products (the "End User"). The transfer/sale of products to the End User is expressly conditional upon End User's acceptance of these terms and conditions.
Restrictions on Use. End Users are specifically not authorized to and are forbidden from reselling, transferring or distributing any products either as a stand alone product or as a component of another product. The right to use the products does not, in and of itself, include or carry any right of the End User to any GE Healthcare Bio-Sciences Corp.'s technology or intellectual property other than expressly provided herein. End Users may not use sequence(s) in an attempt to reverse engineer parameters of any of GE Healthcare Bio-Sciences Corp. proprietary products or services.
Disclaimer of Warranties. GE Healthcare Bio-Sciences Corp. provides no warranties to end user (statutory or implied), including without limitation, as to product quality, condition, description, merchantability or fitness for a particular purpose, and all such warranties are hereby expressly disclaimed. GE Healthcare Bio-Sciences Corp. hereby expressly disclaims any warranty regarding results obtained through the use of the products, including without limitation any claim of inaccurate, invalid or incomplete results.
Exclusion of Liability. GE Healthcare Bio-Sciences Corp. and its affiliates shall have no liability to an End User, including, without limitation, for any loss of use or profits, business interruption or any consequential, incidental, special or other indirect damages of any kind, regardless of how caused and regardless of whether an action in contract, tort, strict product liability or otherwise.
DC2402, DC2408, DG4700 TMR-ET, CXR-ET and CC5 dyes are proprietary.
DC2402, DC2408 Allele sequences for one or more of the loci vWA, FGA, D8S1179, D21S11 and D18S51 in allelic ladder mixtures is licensed under U.S. Pat. Nos. 7,087,380 and 7,645,580, Australia Pat. No. 2003200444 and corresponding patent claims outside the US.
DC2402, DC2408 STR loci are the subject of U.S. Pat. No. RE 37,984, German Pat. No. DE 38 34 636 C2 and other patents issued to the Max-Planck-Gesellschaft zur Förderung der Wissenschaften, e.V., Germany.
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