Drug Discovery

Drug Discovery Categories

ADME Assays

Our wide selection of bioluminescent enzyme substrates for proteases and metabolic enzymes such as CYPs and MAO, among others, provide powerful tools for the ADME-Tox screen.

Drug Target Interaction

Live-cell assays to directly measure ligand affinity, permeability, selectivity and residence time.

Methylation Analysis

Kits for use in epigenetic studies evaluating methylation and gene expression. Includes methylation activity assays, DNA purification, methylation-sensitive restriction enzymes, PCR kits, reporter assays and transfection reagents.

Apoptosis Assays

Promega offers multiple assays to assess different mechanisms and biomarkers of apoptosis. The Caspase-Glo® 3/7, 8, and 9 Assays are the gold standards for simple apoptosis detection.

Cell Viability Assays

Easily measure viability in cell culture, 3D microtissues, bacterial cultures and virus-infected cells. Bioluminescent assays with simple "add-mix-measure" protocols and convenient luminescent readout allow you to easily and sensitively compare cell viability data from well-to-well, from plate-to-plate and from day-to-day.

Custom Assay Materials

Research-grade materials based on luciferase reporter systems and HaloTag® technology. Includes NanoBRET™ technology for protein interaction detection.

Cytotoxicity Assays

Promega offers multiple readouts for quantifying cytotoxicity, including luminescent and fluorescent assays that can be multiplexed with apoptosis or viability assays to help determine mechanism of cell death.

Oxidative Stress Assays

Promega offers assays for detecting glutathione, measuring the ratio of reduced to oxidized glutathione and detecting changes in ROS as an indicator of cell health or cell signaling events.

GPCR Assays

Obtain better dynamic range and more biologically relevant data with the easy-to-use GloSensor™ assay format for live cell analysis.

Histone Deacetylase Assays

Simple, "add-mix-measure" luminescent assays for detecting HDAC and Sirtuin enzyme activities.

Inflammation Assay

Caspase-1 is an essential component of the inflammasome. Conversion of the procaspase-1 zymogen into catalytically active caspase-1 results in 1) the processing and release of cytokines, IL-1β and IL-18, and 2) pyroptosis, an immunogenic form of cell death. Promega introduces a homogenous, bioluminescent method to selectively measure caspase-1 activity directly from cells or medium.

Energy Metabolism Assays

Glucose is the primary energy source for mammalian cells, and NAD(P)H molecules are important cofactors for many enzymes involved in key cellular pathways. Quantitation of these two molecules can determine metabolic activity, including for cells affected by diabetes or cancer.

Protease Assays

Bioluminescent, homogeneous protease assays provide rapid, simple and sensitive measurement of protease activities directly from the cellular environment. Maximum sensitivity can be reached within 10–30 minutes of reagent addition, making these assays ideal for HTS applications.

Protein Interactions

NanoBiT® complementation reporter for protein interactions, prebuilt and do-it-yourself BRET-based assays, protein pulldown systems, and HaloCHIP™--the fast alternative to chromatin immunoprecipitation.

Kinase Assays

Count on minimal interference from screening your compound library with luminescent, ATP-based kinase assays. These sensitive and reliable assays are easily scaled to meet your throughput needs.

Reporter Vectors and Cell Lines

Rapidly develop sensitive reporter assays using robust reporter vectors, luciferase reporter genes and cell lines integrated with luciferase reporter technology.

Target Deconvolution for Early-Phase Drug Discovery

Target identification service using live-cell capture method. Includes low-affinity and low-abundance proteins.