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The SV 96 Total RNA Isolation System provides a high-throughput technique to prepare intact RNA from tissue and cultured cells. Total RNA can be purified from 96 samples in less than an hour without centrifugation. The system also incorporates a DNase treatment step that is designed to substantially reduce genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without phenol:chloroform extraction or ethanol precipitation, and there is no detectable DNase carryover in the final RNA preparation.
Protocols are available for Beckman Coulter and PerkinElmer ...
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Protocols are available for Beckman Coulter and PerkinElmer instruments.
For more information, see the Protocols & Applications Guide.
The SV 96 Total RNA Isolation System requires a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).
For automated total RNA purification using the Biomek® 2000 Laboratory Automation Workstation, the Vacuum Manifold (Beckman Coulter Part# 609670) and Collar (Beckman Coulter Part# 609597) are required. See the Automated Protocol, #EP003, for additional information regarding automated RNA purification.
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SV 96 Total RNA Isolation System
RNA Lysis Buffer (RLA)
RNA Wash Solution (RWA)
DNase Stop Solution (DSA)
Yellow Core Buffer
DNase I (lyophilized)
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This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
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Helix Cabinet, Freezer, Refrigerator
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.
Wizard® SV 96 Binding Plates
Store the SV RNA Lysis Buffer with β-Mercaptoethanol (BME) added at 4°C. Store all other components at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
Z3500, Z3505, Z3051, Z3091, P1195, A2271 For Research Use Only. Not for Use in Diagnostic Procedures.
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