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Cat.# Z5200 contains sufficient reagents for 3 separate mRNA isolations, each from 1–5mg of total RNA. Cat.# Z5210 contains the same reagents as Cat.# Z5200, excluding the Magnetic Separation Stand. Cat.# Z5300 contains sufficient reagents for 15 separate mRNA isolations, each from 100–1,000μg of total RNA. Cat.# Z5310 contains the same reagents as Cat.# Z5300, excluding the Magnetic Separation Stand.
The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in s...
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The PolyATtract® mRNA Isolation Systems use the MagneSphere® technology to isolate mRNA rapidly and effectively from total RNA. The systems use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3´ poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. The mRNA is eluted from the solid phase by the simple addition of ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. The isolated mRNA is suitable for all molecular biology applications, including in vitro translation and cDNA synthesis.
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PolyATtract® mRNA Isolation System I (Refill for Z5200)
Biotinylated Oligo(dT) Probe
mRNA User Tube
Streptavidin MagneSphere® Paramagnetic Particles
PolyATtract® mRNA Isolation System II with Magnetic Stand
MagneSphere® Technology Magnetic Separation Stand (two-position)
PolyATtract® mRNA Isolation System III with Magnetic Stand
PolyATtract® mRNA Isolation System IV (Refill for Z5300)
Biotinylated Oligo(dT) Probe (50pmol/μl)
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This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.
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Store at 4°C. Do not freeze the MagneSphere® Paramagnetic Particles.
For product intended use please see Patents & Disclaimers tab.
Z5210, Z5200, Z5300, Z5310, Z5261, Z5332, Z5333 For Research Use Only. Not for Use in Diagnostic Procedures.
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