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The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion. The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.
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For more information, see the Protocols & Applications Guide.
Cat.# A2258 is supplied with the components listed for Cat.# A2250 except for Alkaline Protease Solution, Nuclease-Free Water, 96-Well Deep Well Plates, Elution Plates and Plate Sealers.
The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).
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Wizard® SV 96 Plasmid DNA Purification System
Alkaline Protease Solution
Wizard® SV 96 Neutralization Solution
Wizard® SV 96 Lysate Clearing Plate
96-Well Deep Well Plate
Wizard® SV 96 Cell Resuspension Solution
Wizard® SV 96 Cell Lysis Solution
Column Wash Solution (CWA)
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
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Wizard® SV 9600 Plasmid DNA Purification System
Wizard® SV 96 Lysate Clearing Plates
Wizard® SV 96 Binding Plates
Column Wash Solution (CWA)
Helix Cabinet, Freezer, Refrigerator
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet, a –20°C Helix Freezer or a +4°C Helix Refrigerator. The program offers numerous convenient solutions to meet your lab's needs.
Store at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
A2250, A2255, A2258, A1318, A1481, A1488, A7113, A7118, A7123, A7128, P1195, A1441, A2271, A2278, A2241, A2248 For Research Use Only. Not for Use in Diagnostic Procedures.A1311 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
A2250, A2255, A2258 U.S. Pat. No. 5,981,235 and other patents and patents pending.
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