Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems

The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion. The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

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The Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems provide a simple and reliable method for the rapid isolation of plasmid DNA using a silica-membrane, 96-well, high-throughput format. A single plate can be processed in 60 minutes or less. The purified plasmid can be used directly for automated fluorescent DNA sequencing as well as for other standard molecular biology techniques, including restriction enzyme digestion. The Wizard® SV 96 and SV 9600 Systems are designed for use either in a manual format or with Beckman Coulter or PerkinElmer automated instruments.

Features - Benefits

• Performance by Design: Vac-Man® 96 Vacuum Manifold eliminates waste handling and allows simultaneous lysate clearing and DNA binding. Novel plate design prevents cross-contamination during sample processing.
• Flexibility: Designed for use in both manual and automated formats.
• Confidence in Results: Purified DNA meets a target of >98% accuracy over 600 bases using pGEM®-3Zf(+) Vector DNA in BigDye® terminator sequencing.
• Automation: Validated automated methods available at: www.promega.com/automethods/
• Your Choice of Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

For more information, see the Protocols & Applications Guide.

Notes

Cat.# A2258 is supplied with the components listed for Cat.# A2250 except for Alkaline Protease Solution, Nuclease-Free Water, 96-Well Deep Well Plates, Elution Plates and Plate Sealers.

The Wizard® SV 96 and Wizard® SV 9600 Systems require a vacuum pump capable of generating 15–20 inches of Hg or equivalent and the Vac-Man® 96 Vacuum Manifold (Cat.# A2291; see figures) or similar manifold. The Vac-Man® 96 Vacuum Manifold requires the use of an external vacuum trap (not provided).

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