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Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Midiprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Midiprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.
The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method fo...
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The PureYield™ Plasmid Midiprep System is designed to isolate transfection-quality plasmid DNA. The system provides a rapid method for purification of 100–200μg of plasmid DNA from 50ml bacteria culture. Plasmid DNA can be purified in as little as 30 minutes with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. An alternative protocol allows purification of over 400μg of high-copy-number plasmid from 250ml of E. coli culture.
The PureYield™ Plasmid Midiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA or extensive centrifugation, providing rapid purification from a single method.
The system has been designed for use with centrifugation or vacuum (e.g., the Vac-Man® Laboratory Vacuum Manifold).
The Eluator™ Vacuum Elution Device is used to elute nucleic acids from PureYield™ Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok® fittings, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield™ Midiprep or Maxiprep columns.
The Eluator™ Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols.
For more information, see the Protocols & Applications Guide.
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PureYield™ Plasmid Midiprep System
Neutralization Solution (NSB)
PureYield™ Binding Columns
PureYield™ Clearing Columns
Endotoxin Removal Wash
Cell Resuspension Solution (CRA)
Cell Lysis Solution (CLA)
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
Already a Helix Customer?
PureYield™ Plasmid Midiprep Start-Up Kit, 110V Electrical (100 preps, manifold, 4 elution devices & free vacuum pump)
Eluator™ Vacuum Elution Device
Welch Vacuum Pump for North America Electrical
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity
PureYield™ Plasmid Midiprep Start-Up Kit, 220V Electrical (100 preps, manifold, 4 elution devices & free vacuum pump)
Welch Vacuum Pump for Continental Europe Electrical
PureYield™ Plasmid Midiprep Start-Up Kit, 230V Electrical (100 preps, manifold, 4 elution devices & free vacuum pump)
Welch Vacuum Pump for Japan Electrical
Store all system components at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
Elution is completed using vacuum pressure with a Vac-Man® Laboratory Manifold in less than one minute directly into 1.5ml centrifuge tubes.
A2492, A2495, A2496, A6740, A6742, A6744, A7115, A7125, A1485, A1071 For Research Use Only. Not for Use in Diagnostic Procedures.
A2492, A2495, A2496 U.S. Pat. No. 6,194,562 and other patents pending.
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