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Special Offer: Switch to the Vacuum Format and get a Free Welch® Vacuum Pump! For a limited time PureYield™ Plasmid Maxiprep Start-Up Kits are available. Purchase a PureYield™ Plasmid Maxiprep System and a Vac-Man® Vacuum Manifold, and get a free Welch® Vacuum Pump! Each vacuum pump bundle is available in 3 formats. If you are not sure which format is right for your geographic area, contact your Promega representative or an authorized Promega distributor.
The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification...
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The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods.
The PureYield™ Plasmid Maxiprep System incorporates a unique Endotoxin Removal Wash designed to remove substantial amounts of protein, RNA and endotoxin contaminants from purified plasmid DNA. Removal of contaminants improves the robustness of sensitive applications such as eukaryotic transfection, in vitro transcription and coupled in vitro transcription/translation (e.g., TnT® Quick Coupled Transcription/Translation System). Purification is achieved without isopropanol precipitation of purified plasmid DNA.
The system has been designed for use with a vacuum source and vacuum manifold (e.g., the Vac-Man® Laboratory Vacuum Manifold).
The Eluator™ Vacuum Elution Device is used to elute nucleic acids from PureYield™ Midiprep or Maxiprep columns. It consists of two pieces, a blue base and a clear column assembly. The base interfaces with a Vacuum Manifold that contains Luer-Lok® fittings, such as the Vac-Man® Laboratory Vacuum Manifold (Cat.# A7231), and holds a 1.5ml tube to capture the eluted nucleic acids. The column assembly accepts PureYield™ Midiprep or Maxiprep columns.
The Eluator™ Device eliminates the requirement for a centrifuge with a swinging bucket rotor for nucleic acid purification, simplifying and speeding purification protocols.
For more information, see the Protocols & Applications Guide.
This product requires the use of a vacuum pump and vacuum manifold.
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PureYield™ Plasmid Maxiprep System
Neutralization Solution (NSB)
PureYield™ Clearing Columns
PureYield™ Maxi Binding Columns
Endotoxin Removal Wash
Cell Resuspension Solution (CRA)
Cell Lysis Solution (CLA)
This product can be added to a
This product is available through the Promega Helix onsite stocking program in a room temperature cabinet. The program offers numerous convenient solutions to meet your lab's needs. The Helix Cabinet is available in two sizes: 7.9 cubic ft. or 25 cubic ft.
Already a Helix Customer?
PureYield™ Plasmid Maxiprep Start-Up Kit, 110V Electrical (50 preps, manifold, 4 elution devices and free vacuum pump)
Eluator™ Vacuum Elution Device
Welch Vacuum Pump for North America Electrical
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity
PureYield™ Plasmid Maxiprep Start-Up Kit, 220V Electrical (50 preps, manifold, 4 elution devices and free vacuum pump)
Welch Vacuum Pump for Continental Europe Electrical
PureYield™ Plasmid Maxiprep Start-Up Kit, 230V Electrical (50 preps, manifold, 4 elution devices and free vacuum pump)
Welch Vacuum Pump for Japan Electrical
Store at 22–25°C.
For product intended use please see Patents & Disclaimers tab.
Elution is completed using vacuum pressure with a Vac-Man® Laboratory Manifold in less than one minute directly into 1.5ml centrifuge tubes.
A2392, A2393, A6730, A6732, A6734, A1071 For Research Use Only. Not for Use in Diagnostic Procedures.
A2392, A2393 U.S. Pat. No. 6,194,562 and other patents pending.
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