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Promega Corporation

Universal RiboClone® cDNA Synthesis System

The Universal RiboClone® cDNA Synthesis System contains the reagents required for synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector. The system is based on the method described by Okayama and Berg with modifications by Gubler and Hoffman. First-strand synthesis is driven by AMV (Avian Myeloblastosis Virus) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer, followed directly by second-strand replacement synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to flush the ends, the double-stranded cDNA molecules are p...

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  • Prices valid for US customers only
Product Size Conc. Catalog # *List Price QTY Add to Cart

Universal RiboClone® cDNA Synthesis System

1 system
- C4360 $ 644.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

Oligo(dT)15 Primer

20μg
- C1101 $ 57.00 Add to cart

Random Primers

20μg
- C1181 $ 37.00 Add to cart

Spin Columns

10 each
- C1281 $ 45.00 Add to cart

EcoRI Adaptors

150pmol
- C1291 $ 45.00 Add to cart

1.2kb Kanamycin Positive Control RNA

5μg
- C1381 $ 65.00 Add to cart

Sephacryl® S-400

10ml
- V3181 $ 82.00 Add to cart

Storage Conditions

Store control RNA at –70°C. Store Sephacryl® S-400 at 4°C and Spin Columns at room temperature. Store other components at –20°C.

For product intended use please see Patents & Disclaimers tab.

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Scientists at Your Service

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