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Promega Corporation

Universal RiboClone® cDNA Synthesis System

The Universal RiboClone® cDNA Synthesis System contains the reagents required for synthesis of double-stranded cDNA from mRNA and subsequent ligation into a suitable vector. The system is based on the method described by Okayama and Berg with modifications by Gubler and Hoffman. First-strand synthesis is driven by AMV (Avian Myeloblastosis Virus) Reverse Transcriptase and either Random Primers or Oligo(dT)15 Primer, followed directly by second-strand replacement synthesis using RNase H and DNA Polymerase I. After treatment with T4 DNA Polymerase to flush the ends, the double-stranded cDNA molecules are p...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Universal RiboClone® cDNA Synthesis System

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Universal RiboClone® cDNA Synthesis System

 Components

  • Sodium Pyrophosphate

    C113A1 x 50μl
  • First Strand 5X Buffer

    C121A1 x 200μl
  • T4 DNA Ligase 10X Buffer

    C126A1 x 500μl
  • Spin Columns

    C12811 x 10 each
  • EcoRI Adaptors

    C129A1 x 150pmol
  • Kinase 10X Buffer

    C131A1 x 500μl
  • ATP

    C134A1 x 50μl
  • 1.2kb Kanamycin Positive Control RNA

    C138B1 x 10μg
  • Oligo(dT)15 Primer

    C144A1 x 20μg
  • Random Primers

    C146A1 x 20μg
  • Second Strand 2.5X Buffer, AMV

    C630A1 x 800μl
  • T4 DNA Ligase

    M180A1 x 100u
  • DNA Polymerase I

    M205A1 x 500u
  • T4 Polynucleotide Kinase

    M410A1 x 100u
  • T4 DNA Polymerase

    M421A1 x 100u
  • RNase H

    M428B1 x 20u
  • AMV Reverse Transcriptase (HC)

    M900A1 x 600u
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251E1 x 1,000u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • Acetylated BSA

    R946A1 x 400μl
  • Sephacryl® S-400

    V318A1 x 10ml
1 system
- C4360 $ 644.00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Oligo(dT)15 Primer

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Oligo(dT)15 Primer

 Components

  • Oligo(dT)15 Primer

    C110A1 x 20μg
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


20μg
- C1101 $ 57.00 Add to cart

Random Primers

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Random Primers

 Components

  • Random Primers

    C118A1 x 20μg
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


20μg
- C1181 $ 37.00 Add to cart

Spin Columns

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Spin Columns

 Components

10 each
- C1281 $ 45.00 Add to cart

EcoRI Adaptors

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EcoRI Adaptors

 Components

  • EcoRI Adaptors

    C129A1 x 150pmol
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


150pmol
- C1291 $ 45.00 Add to cart

1.2kb Kanamycin Positive Control RNA

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1.2kb Kanamycin Positive Control RNA

 Components

  • 1.2kb Kanamycin Positive Control RNA

    C138A1 x 5μg
5μg
- C1381 $ 65.00 Add to cart

Sephacryl® S-400

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Sephacryl® S-400

 Components

  • Sephacryl® S-400

    V318A1 x 10ml
10ml
- V3181 $ 82.00 Add to cart

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Storage Conditions

Store control RNA at –70°C. Store Sephacryl® S-400 at 4°C and Spin Columns at room temperature. Store other components at –20°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

C4360, C1101, C1181, C1281, C1291, C1381, V3181 For Research Use Only. Not for Use in Diagnostic Procedures.

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