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Promega Corporation

Terminal Deoxynucleotidyl Transferase, Recombinant

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Terminal Deoxynucleotidyl Transferase, Recombinant, catalyzes the repetitive addition of mononucleotides to the terminal 3´-OH of a DNA initiator accompanied by the release of inorganic phosphate. Single-stranded DNA is preferred as an initiator. Polymerization is not template-dependent. The addition of 1mM Co2+ (as CoCl2) in the reaction buffer allows the tailing of 3´-ends with varying degrees of efficiency.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Terminal Deoxynucleotidyl Transferase, Recombinant

300u
30u/μl M1871 $ 80.00 Add to cart

Terminal Deoxynucleotidyl Transferase, Recombinant

1,500u
30u/μl M1875 $ 280.00 Add to cart


Available Separately

Product Size Conc. Catalog # *List Price QTY Add to Cart

Terminal Transferase Buffer Pack

3 × 500μl
- M1893 $ 20.00 Add to cart

QC Tests

Activity, endonuclease, DNase, RNase, 3´ end-labeling, apoptotic DNA end-labeling.

Source

Recombinant E. coli strain.

Storage Buffer

50mM potassium phosphate (pH 6.4), 100mM NaCl, 1mM β-mercaptoethanol, 0.1% Tween® 20 and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit of activity catalyzes the transfer of 0.5 picomoles of ddATP to oligo(dT)16 per minute at 37°C in 1X Terminal Transferase Buffer. The resulting oligo(dT)17 is measured by HPLC.

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