Activity, endonuclease, DNase, RNase, 3´ end-labeling, apoptotic DNA end-labeling.
Recombinant E. coli strain.
50mM potassium phosphate (pH 6.4), 100mM NaCl, 1mM β-mercaptoethanol, 0.1% Tween® 20 and 50% glycerol.
Store at –20°C.
One unit of activity catalyzes the transfer of 0.5 picomoles of ddATP to oligo(dT)16 per minute at 37°C in 1X Terminal Transferase Buffer. The resulting oligo(dT)17 is measured by HPLC.
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