T4 RNA Ligase

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5´-³²P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA.

Expand to Read More »

T4 RNA Ligase catalyzes the ATP-dependent ligation of single-stranded RNA or DNA onto the 5´-phosphoryl termini of single-stranded RNA or DNA. The enzyme, purified from recombinant E. coli CA4 (RNase I-deficient), has an apparent molecular weight of 43.5kDa. T4 RNA Ligase also catalyzes the addition of [5´-³²P] nucleoside 3´,5´-bis (phosphate) onto single-stranded RNA.

Features - Benefits

• May Be Heat-Inactivated: T4 RNA Ligase may be inactivated by heating at 65°C for 15 minutes.
• Provided with 10X Reaction Buffer: 500mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl₂, 50mM DTT, 10mM ATP.

Applications

• Ligation of single-stranded nucleic acids.
• 3´ end-labeling of RNA.
• Circularization of RNA and DNA oligonucleotides.
• Cloning full-length cDNA.

References

1. Silber, R. et al. (1972) Proc. Natl. Acad. Sci. USA 69, 3009–13.
2. England, T. et al. (1977) Proc. Natl. Acad. Sci. USA 74, 4839–42.
3. Uhlenbeck, O.C. and Gumport, R.I. (1982) The Enzymes 158, 31.
4. Brennan, C.A. et al. (1983) Meth. Enzymol. 100, 38–52.
5. Schaefer, B.C. (1995) Anal. Biochem. 227, 255–73.

« Collapse Content