T4 Polynucleotide Kinase

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T4 Polynucleotide Kinase Protocol MSDS Components Close Window T4 Polynucleotide Kinase  Components Kinase 10X Buffer C131B1 x 100μl T4 Polynucleotide Kinase M410A1 x 100u	100u	5–10u/μl	M4101	$ 54.00		Add to cart
T4 Polynucleotide Kinase Protocol MSDS Components Close Window T4 Polynucleotide Kinase  Components Kinase 10X Buffer C131A2 x 500μl T4 Polynucleotide Kinase M410B1 x 1,000u	1,000u	5–10u/μl	M4103	$ 394.00		Add to cart

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QC Tests

Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-³²P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl₂, 5mM DTT, 0.1mM [γ-³²P] ATP and 0.5mM 5´-OH polynucleotide end concentration.

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Use Restrictions

M4101, M4103, C1313 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.