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Promega Corporation

T4 Polynucleotide Kinase

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T4 Polynucleotide Kinase catalyzes the transfer of the γ-phosphate from ATP to the 5´-terminus of polynucleotides or to mononucleotides bearing a 5´-hydroxyl group. The enzyme, purified from recombinant E. coli, may be used to phosphorylate RNA, DNA and synthetic oligonucleotides prior to subsequent manipulations such as ligation.

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T4 Polynucleotide Kinase

100u
5–10u/μl M4101 $ 55.00 Add to cart

T4 Polynucleotide Kinase

1,000u
5–10u/μl M4103 $ 403.00 Add to cart


Available Separately

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T4 PNK Buffer Pack

1.5ml
(3 × 500μl)
- C1313 $ 28.00 Add to cart

QC Tests

Activity, DNase, RNase, endonuclease, end-labeling, blue/white cloning assay.

Source

Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5), 25mM KCl, 2mM DTT, 0.1mM EDTA, 0.1μM ATP and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the transfer of 1nmol of phosphate to the 5´-OH end of a polynucleotide from [γ-32P]ATP in 30 minutes at 37°C. The reaction conditions are: 40mM Tris-HCl (pH 7.5 at 25°C), 10mM MgCl2, 5mM DTT, 0.1mM [γ-32P] ATP and 0.5mM 5´-OH polynucleotide end concentration.

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