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Promega Corporation

T4 DNA Polymerase

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T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

T4 DNA Polymerase

100u
5–10u/μl M4211 $ 97.00 Add to cart

T4 DNA Polymerase

500u
5–10u/μl M4215 $ 380.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease.

Source

Recombinant E. coli strain.

Storage Buffer

200mM potassium phosphate (pH 6.5 at 25°C), 2mM DTT and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.

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