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Promega Corporation

T4 DNA Polymerase

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T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs.

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T4 DNA Polymerase

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T4 DNA Polymerase

 Components

  • T4 DNA Polymerase

    M421A1 x 100u
  • T4 DNA Polymerase 10X Reaction Buffer

    M831A1 x 1ml
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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100u
5–10u/μl M4211 $ 97.00 Add to cart

T4 DNA Polymerase

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T4 DNA Polymerase

 Components

  • T4 DNA Polymerase

    M421F1 x 500u
  • T4 DNA Polymerase 10X Reaction Buffer

    M831A1 x 1ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500u
5–10u/μl M4215 $ 380.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease.

Source

Recombinant E. coli strain.

Storage Buffer

200mM potassium phosphate (pH 6.5 at 25°C), 2mM DTT and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 55nmol of dTTP into acid-precipitable material in 30 minutes at 39°C using poly(dA):oligo(dT) as a substrate.

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