T4 DNA Polymerase

T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs.

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T4 DNA Polymerase catalyzes the 5´→3´ synthesis of DNA from a primed single-stranded DNA template. Although possessing a potent 3´→5´ proofreading exonuclease, T4 DNA Polymerase contains no 5´→3´ exonuclease activity. T4 DNA Polymerase can be used to fill 5´ protruding ends with labeled or unlabeled dNTPs or for the generation of blunt ends from DNA molecules with 3´ overhangs.

Features - Benefits

• High Fidelity: T4 DNA Polymerase is the enzyme of choice for applications where misincorporation is a concern.
• Flexible: T4 DNA Polymerase may be used in a variety of molecular applications. Active in many Promega 1X restriction enzyme buffers.
• May Be Heat-Inactivated: T4 DNA Polymerase is inactivated by heating at 75°C for 10 minutes.
• Provided with 10X Reaction Buffer: 250mM Tris-acetate (pH 7.7), 1M potassium acetate, 100mM magnesium acetate and 10mM DTT.
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Flush 5´ protruding ends with labeled or unlabeled dNTPs.
• Blunt 3´ overhangs.
• In vitro mutagenesis.

References

1. Challberg, M.D. and Englund, P.T. (1980) Meth. Enzymol. 65, 39–43.
2. Burd, J.F. and Wells, R.D. (1974) J. Biol. Chem. 249, 7094–801.
3. Kunkel, T.A., Loeb, L.A. and Goodman, M.F. (1984) J. Biol. Chem. 259, 1539–45.

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