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Promega Corporation

T3 RNA Polymerase

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T3 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only T3 DNA or DNA cloned downstream from a T3 promoter can serve as a template for T3 RNA Polymerase-directed RNA synthesis.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

T3 RNA Polymerase

10–20u/μl P2083 $ 51.00 Add to cart

T3 RNA Polymerase (HC)

80u/μl P4024 $ 101.00 Add to cart

QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.


Recombinant E. coli strain.

Storage Buffer

20mM potassium phosphate (pH 7.7 at 25°C), 1.0mM EDTA, 10mM DTT, 100mM NaCl, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP and 2μg supercoiled pSP6/T3 vector DNA.

For product intended use please see Patents & Disclaimers tab.

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