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Promega Corporation

SP6 RNA Polymerase

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SP6 RNA Polymerase is a DNA-dependent RNA polymerase that exhibits extremely high specificity for its cognate promoter sequences. Only SP6 DNA or DNA cloned downstream from an SP6 promoter can serve as a template for SP6 RNA Polymerase-directed RNA synthesis.

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

SP6 RNA Polymerase

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SP6 RNA Polymerase

 Components

  • SP6 RNA Polymerase

    P108B1 x 1,000u
  • DTT

    P117B1 x 250μl
  • Transcription Optimized 5X Buffer

    P118B1 x 500μl
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  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


1,000u
10–20u/μl P1085 $ 210.00 Add to cart

SP6 RNA Polymerase

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SP6 RNA Polymerase

 Components

  • SP6 RNA Polymerase

    P108G1 x 5,000u
  • DTT

    P117C1 x 1.25ml
  • Transcription Optimized 5X Buffer

    P118C2 x 1.25ml
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


5,000u
10–20u/μl P1081 $ 708.00 Add to cart

SP6 RNA Polymerase (HC)

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SP6 RNA Polymerase (HC)

 Components

  • DTT

    P117B1 x 250μl
  • Transcription Optimized 5X Buffer

    P118B1 x 500μl
  • SP6 RNA Polymerase (HC)

    P408A1 x 2,500u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


2,500u
80u/μl P4084 $ 405.00 Add to cart


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QC Tests

Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, transcription.

Source

Recombinant E. coli strain.

Storage Buffer

20mM potassium phosphate buffer (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% (v/v) Triton® X-100 and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to catalyze the incorporation of 5nmol of CTP into acid-insoluble product in 60 minutes at 37°C in a total volume of 100μl. The reaction conditions are: 40mM Tris-HCl (pH 7.9 at 25°C), 6mM MgCl2, 10mM DTT, 10mM NaCl, 2mM spermidine, 0.05% Tween® 20, 0.5mM each of ATP, GTP, CTP, and UTP, 0.5μCi [3H]CTP, and 2μg supercoiled pGEM®-5Zf(+) Vector DNA.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

P1085, P1081, P4084 For Research Use Only. Not for Use in Diagnostic Procedures.

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