Activity, unidirectional deletions using the Erase-a-Base™ System.
Fungal α amylase powder.
20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl2, 50mM NaCl and 50% (v/v) glycerol.
Store at –20°C.
One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.
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