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Promega Corporation

S1 Nuclease

S1 Nuclease degrades single-stranded DNA and RNA endonucleolytically to yield 5´-phosphoryl-terminated products. Double-stranded nucleic acids (DNA:DNA, DNA:RNA or RNA:RNA) are resistant to degradation except with extremely high concentrations of enzyme. The enzyme is used to remove single-stranded termini from double-stranded DNA, for selective cleavage of single-stranded DNA and for mapping RNA transcripts.

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S1 Nuclease

10,000u
20–100u/μl M5761 $ 40.00 Add to cart


QC Tests

Activity, unidirectional deletions using the Erase-a-Base™ System.

Source

Fungal α amylase powder.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 0.1mM ZnCl2, 50mM NaCl and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1μg of acid-soluble material per minute at 37°C in 30mM sodium acetate (pH 4.6 at 25°C), 50mM NaCl, 1mM ZnCl2, 0.5mg/ml denatured calf thymus DNA and 5% glycerol.

For product intended use please see Patents & Disclaimers tab.

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