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Promega Corporation

RNase ONE™ Ribonuclease

RNase ONE™ Ribonuclease is a 27kDa periplasmic enzyme from E. coli that catalyzes the degradation of RNA to cyclic nucleotide monophosphate (NMP) intermediates. Slower hydrolysis further catalyzes the degradation of these intermediates to 3´-NMPs. RNase ONE™ Ribonuclease is one of the few known RNases that can cleave a phosphodiester bond between any two ribonucleotides. RNase ONE™ Ribonuclease may be used to remove RNA from DNA preparations, for RNase protection assays and for mapping or quantitation of RNA by selective cleavage of single-stranded regions.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

RNase ONE™ Ribonuclease

1,000u
5–10u/μl M4261 $ 114.00 Add to cart

RNase ONE™ Ribonuclease

5,000u
5–10u/μl M4265 $ 374.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease, DNase.

Source

Recombinant E. coli strain.

Storage Buffer

10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C. Do not freeze at –70°C. Do not store on dry ice.

Unit Definition

One unit is defined as the amount of enzyme required to completely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.

For product intended use please see Patents & Disclaimers tab.

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