Activity, SDS-PAGE/purity, endonuclease, DNase.
Recombinant E. coli strain.
10mM Tris-HCl (pH 8.0), 200mM NaCl, 50% (v/v) glycerol.
Store at –20°C. Do not freeze at –70°C. Do not store on dry ice.
One unit is defined as the amount of enzyme required to
completely degrade RNA at the rate of 100ng/second at 37°C in 10mM Tris-HCl (pH 7.5 at 25°C), 5mM EDTA, 200mM sodium acetate, 0.2μg/μl RNA, 0.05% NP-40 and 2μg/μl BSA.
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