Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.
10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.
Store at –20°C.
0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.
For product intended use please see Patents & Disclaimers tab.