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Promega Corporation

HQ and GST Tag Flexi® Vectors for E. coli and Cell-Free Protein Expression

The vectors below are used for inducible expression of HQ- and GST-tagged fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. The HQ tag and polyhistidine tag (His) are comparable in their affinity for Ni ions and will bind to all His-binding surfaces and resins. In certain cases the HQ-tagged proteins can be eluted from the affinity columns at lower concentrations of imidazole—a property useful for some downstream applications such as enzymatic reactions. As with His tag, proteins can be expressed from bacterial, insect and mammalian systems and purified under either native or...

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pFN2A (GST) Flexi® Vector

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pFN2A (GST) Flexi® Vector

 Components

  • pFN2A (GST) Flexi® Vector

    C846A1 x 20μg
20μg
- C8461 $ 298.00 Add to cart

pFN2K (GST) Flexi® Vector

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pFN2K (GST) Flexi® Vector

 Components

  • pFN2K (GST) Flexi® Vector

    C847A1 x 20μg
20μg
- C8471 $ 298.00 Add to cart

pFN6A (HQ) Flexi® Vector

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pFN6A (HQ) Flexi® Vector

 Components

  • pFN6A (HQ) Flexi® Vector

    C851A1 x 20μg
20μg
- C8511 $ 298.00 Add to cart

pFN6K (HQ) Flexi® Vector

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pFN6K (HQ) Flexi® Vector

 Components

  • pFN6K (HQ) Flexi® Vector

    C852A1 x 20μg
20μg
- C8521 $ 298.00 Add to cart

pFC7A (HQ) Flexi® Vector

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pFC7A (HQ) Flexi® Vector

 Components

  • pFC7A (HQ) Flexi® Vector

    C853A1 x 20μg
20μg
- C8531 $ 298.00 Add to cart

pFC7K (HQ) Flexi® Vector

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pFC7K (HQ) Flexi® Vector

 Components

  • pFC7K (HQ) Flexi® Vector

    C854A1 x 20μg
20μg
- C8541 $ 298.00 Add to cart

pF1A T7 Flexi® Vector

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pF1A T7 Flexi® Vector

 Components

  • pF1A T7 Flexi® Vector

    C844A1 x 20μg
20μg
- C8441 $ 263.00 Add to cart

pF1K T7 Flexi® Vector

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pF1K T7 Flexi® Vector

 Components

  • pF1K T7 Flexi® Vector

    C845A1 x 20μg
20μg
- C8451 $ 263.00 Add to cart


GenBank®/EMBL Accession Number

pFN2A (GST) Accession Number AY753578; pFN2A (GST) Vector sequence text file.
pFN2K (GST) Accession Number AY753579; pFN2K (GST) Vector sequence text file.
pFN6A (HQ) Accession Number, DQ132629; pFN6A (HQ) Vector sequence text file.
pFN6K (HQ) Accession Number DQ132630; pFN6K (HQ) Vector sequence text file.
pFC7A (HQ) Accession Number DQ133906; pFC7A (HQ) Vector sequence text file.
pFC7K (HQ) Accession Number DQ133907; pFC7K (HQ) Vector sequence text file.
pF1A T7 Accession Number AY753576; pF1A T7 Vector sequence text file.
pF1K T7 Accession Number AY753577; pF1K T7 Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

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