Activity, SDS-PAGE/purity, endonuclease, 3´ overhang protection assay.
Recombinant E. coli strain.
20mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% (v/v) glycerol.
Store at –20°C.
One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble nucleotides from double-stranded DNA in 30 minutes at 37°C.
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