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Promega Corporation

Exonuclease III

Exonuclease III is a 3´→5´ exonuclease specific for double-stranded DNA. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. Exonuclease III will also degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase activity. In addition, the enzyme has apurinic endonuclease activity and ribonuclease H activity. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments.

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Product Size Conc. Catalog # *List Price QTY Add to Cart

Exonuclease III

150–200u/μl M1811 $ 89.00 Add to cart

Exonuclease III

150–200u/μl M1815 $ 287.00 Add to cart

QC Tests

Activity, SDS-PAGE/purity, endonuclease, 3´ overhang protection assay.


Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble nucleotides from double-stranded DNA in 30 minutes at 37°C.

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