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DNA Polymerase I Large (Klenow) Fragment

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DNA Polymerase I Large (Klenow) Fragment is a DNA-dependent DNA polymerase that lacks the 5´→3´ exonuclease activity of intact E. coli DNA Polymerase I but retains its 5´→3´ polymerase, 3´→5´ exonuclease and strand displacement activities. The enzyme is a 68kDa C-terminal fragment of DNA Polymerase I. The 5´→3´ polymerase activity of Klenow Fragment can be used to fill in 5´-protruding ends with unlabeled or labeled dNTPs, to sequence single- or double-stranded DNA templates, for in vitro mutagenesis using synthetic oligonucleotides, for cDNA second-strand synthesis and to generate single-stranded DNA probes. The ...

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DNA Polymerase I Large (Klenow) Fragment

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DNA Polymerase I Large (Klenow) Fragment

 Components

  • DNA Polymerase 10X Buffer

    M195A1 x 1ml
  • DNA Polymerase I Large (Klenow) Fragment

    M220A1 x 150u
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  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


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150u
5u/μl M2201 $ 110.00 Add to cart

DNA Polymerase I Large (Klenow) Fragment

Close Window

DNA Polymerase I Large (Klenow) Fragment

 Components

  • DNA Polymerase 10X Buffer

    M195A1 x 1ml
  • DNA Polymerase I Large (Klenow) Fragment

    M220C1 x 500u
Close Window
  • This product can be added to a

  • Helix Freezer

This product is available through the Promega Helix onsite stocking program in a –20°C Helix Freezer. The program offers numerous convenient solutions to meet your lab's needs. Helix Freezers are available in two sizes: 5.7 cubic ft. or 9.7 cubic ft.


Click here to learn more about the Helix Collection »


Already a Helix Customer?

Request to add this product to your Helix »


500u
5u/μl M2206 $ 267.00 Add to cart


QC Tests

Activity, SDS-PAGE/purity, endonuclease.

Source

Recombinant E. coli strain.

Storage Buffer

50mM Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmol of total deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.5 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA and 33μM each of dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).

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