Activity, SDS-PAGE/purity, endonuclease.
Recombinant E. coli strain.
50mM Tris-HCl (pH 7.5), 1mM DTT, 0.1mM EDTA and 50% (v/v) glycerol.
Store at –20°C.
One unit is defined as the amount of enzyme that incorporates 10nmol of total deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.5 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA and 33μM each of dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).
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