Activity, endonuclease, DNase, RNase, blue/white cloning assay.
Calf intestinal mucosa.
10mM Tris-HCl (pH 8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% (v/v) glycerol.
Store at –20°C.
One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).
For product intended use please see Patents & Disclaimers tab.
M1821, M2825, M1833 For Research Use Only. Not for Use in Diagnostic Procedures.