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Bacterial Strain JM109(DE3), derived from JM109, contains a chromosomal copy of the gene for T7 RNA polymerase. JM109(DE3) is used for the high-level expression of genes cloned into vectors for expression of sequence downstream from the T7 promoter, provided that the cloned sequence contains a ribosomal binding site. Please note: JM109(DE3) cannot be used for blue/white selection.
Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F´, traD36, proAB, lacIqZΔM15], lDE3....
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Genotype: endA1, recA1, gyrA96, thi, hsdR17 (rk–, mk+), relA1, supE44, λ–, Δ(lac-proAB), [F´, traD36, proAB, lacIqZΔM15], lDE3.
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Bacterial Strain JM109(DE3), Glycerol Stock
Bacterial Strain JM109(DE3)
Store at –70°C.
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P9801 For Research Use Only. Not for Use in Diagnostic Procedures.
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