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Promega Corporation

Protein Phosphatase Assays

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Protease Assays 4620CA
Highly Sensitive Non-Radioactive Phosphatase Assays

Phosphatases are the opposing partners to the kinases in the cell, catalyzing the dephosphorylation of molecules involved in cellular pathways, and so are important targets for drug screening and basic cell biology research. We have developed non-radioactive assays that provide the high level of sensitvity and reliability (Z´-factor >0.7) required for high-qualilty, reliable data.

Featured Products

ProFluor™ Phosphatase Assays

The ProFluor® Phosphatase Assays overcome safety issues associated with radioactive assays while maintaining sensitivity, specificity and simplicity required for screening chemical libraries for novel inhibitors of protein phosphatases. These assays are robust with Z´ factor values routinely greater than 0.8.

Colorimetric Phosphatase Assays

Both the Tyrosine Phosphatase and the Serine/Threonine Phosphatase Assay Systems detect the release of phosphate from specific peptide substrates by measuring the appearance of a phosphate complex of molybdate:malachite green. Each system includes ready-to-use, specific substrates.

Protein Phosphatases

PPase-2A is a serine/threonine phosphatase isolated from human red blood cells. It is isolated as the heterodimer of 60kDa (A) and 36kDa (C) subunits. It has the ability to dephosphorylate the α-subunit of phosphorylase kinase. PPase-2B is a heterodimeric enzyme composed of a 19kDa calcium-binding subunit and a catalytic subunit (61kDa) that binds calmodulin.

Featured Resources

PAG_CellSignaling_fr Cell Signaling

This chapter of the Protocols and Applications Guide describes the molecular basis of cell signaling and the various assays used to study cell signaling.

Multimedia_fr Culture Preparation and Plating

This video covers a procedure for culturing and plating cells for use in a cell-based assay such as CellTiter-Glo® Luminescent Cell Viability Assay.

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Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

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