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See All Kinase Enzyme Systems
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type CHK2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative CHK2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2).
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures A...
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ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects chemical compounds have on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.
Kinase Enzyme System contains:
CHK2 NCBI Database Entry.
Human Kinase Enzyme Systems and Applications.
Kinase Enzyme System manufactured by SignalChem.
Bulk quantities available upon request.
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CHK2 Kinase Enzyme System
ADP-Glo™ Kinase Assay
Upon receipt, centrifuge the kinase and dispense it into smaller quantities. Store the kinase at –70°C and the rest of the components at –20°C.
For product intended use please see Patents & Disclaimers tab.
V4020, V6284, V4021 For Research Use Only. Not for Use in Diagnostic Procedures.
V4021 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.
V4021 U.S. Pat. No. 7,700,310 and other patents and patents pending.
V4021 U.S. Pat. Nos. 7,741,067, 8,361,739 and 8,603,767 and other patents and patents pending.
V4021 U.S. Pat. Nos. 6,602,677, 7,241,584, 8,030,017 and 8,822,170 and other patents and patents pending.
V4021 U.S. Pat. No. 8,183,007 and other patents and patents pending.
V4021 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.
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