cAMP-Glo™ Assay
Simple, Bioluminescent cAMP Detection
- Complete in ~45 minutes
- Scalable to 1536-well plate formats and beyond
- No interference by fluorescent compounds
Catalog Number:
Size
Catalog Number: V1501
Catalog Number: V1502
Monitor Effect of Test Compounds on GPCRs
The cAMP-Glo™ Assay is designed to monitor cAMP production in response to the effects of test compounds on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.
The cAMP-Glo™ Assay can be performed in 96-, 384- or 1536-well plates. The cells are induced with a test compound for an appropriate period of time to modulate cAMP levels. After induction, cells are lysed to release cAMP, and then the cAMP detection solution, which contains protein kinase A, is added. Kinase-Glo® Reagent is then added to terminate the PKA reaction and detect the remaining ATP via a luciferase reaction. Plates are read using a microplate-reading luminometer. Luminescence can be correlated to cAMP concentrations using a cAMP standard curve. The half-life for the luminescent signal is greater than 4 hours. This extended signal half-life eliminates the need for luminometers with reagent injectors and allows batch-mode processing of multiple plates.
Cat.# V1501 contains sufficient reagents to perform 300 assays at 60μl final volume per well in 384-well plates.
Cat.# V1502 contains sufficient reagents to perform 3,000 assays at 60μl final volume per well in 384-well plates.
Excellent Signal:Background
The assay provides the best signal:background ratio of all the cAMP assays; signal:background >200 (with cAMP), >15 (on cells).Proven Luminescence Technology
Powered by Ultra-Glo™ Recombinant Luciferase, the bioluminescence-based cAMP-Glo™ assay provides a safe alternative to radioactive methods, and avoids the interference issues that can be problematic for fluorescence-based methods.
cAMP production in cells and the cAMP-Glo™ Assay.
Representation of cAMP production in cells and the cAMP-Glo™ Assay.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Protein Kinase A |
V135D | 1 × 16μl |
|
cAMP-Glo™ Lysis Buffer |
V151A | 1 × 5ml |
|
cAMP-Glo™ Reaction Buffer |
V152A | 1 × 8ml |
|
cAMP, 1mM |
V642B | 1 × 100μl |
|
Kinase-Glo® Substrate |
V672A | 1 × 1 vial |
|
Kinase-Glo® Buffer |
V673A | 1 × 10ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 7,700,310 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Protein Kinase A |
V135C | 1 × 160μl |
|
cAMP-Glo™ Lysis Buffer |
V151B | 1 × 25ml |
|
cAMP-Glo™ Reaction Buffer |
V152B | 1 × 50ml |
|
cAMP, 1mM |
V642B | 1 × 100μl |
|
Kinase-Glo® Substrate |
V672B | 1 × 1 vial |
|
Kinase-Glo® Buffer |
V673B | 1 × 100ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. No. 7,700,310 and other patents and patents pending.
Related Products
Similar Products
cAMP-Glo™ Max Assay
30-minute, high-throughput assay for cAMP in cells.
V1681, V1682, V1683
GloSensor™ Technology
Live-cell biosensors for cAMP, cGMP or protease activity.
E1290, E1291, E2301, E1171
cAMP-Dependent Protein Kinase, Catalytic Subunit
Purified kinase for protein phosphorylation and signal transduction studies.
V5161
Frequently Used With
GloMax® Explorer System
Easy-to-use, flexible multimode detection system.
GM3500, GM3510
GloMax® Discover System
High-performance microplate reader for detecting luminescence, fluorescence and absorbance.
GM3000
Kinase-Glo® Luminescent Kinase Assays
Determines activity of purified kinases by quantifying ATP remaining after a kinase reaction.
V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774
