Each chapter includes:
- Step-by-step protocols
- Illustrations and example data
- Extensive background information
- Animated movies of key techniques and cellular events
- Links to citations and other useful laboratory tools
- Introductory animation about PCR
- Optimization tricks
- RT-PCR tips
- Polymerase and transcriptase review
- PCR & RT-PCR protocols
- Troubleshooting
- Learn how to design and optimize siRNA sequences
- Use reporter genes for siRNA optimization
- Investigate RNAi delivery strategies
- View a Nature animation about the RNAi mechanism
- Gather more data from a single sample with multiplexed assays
- Use two or more methods to confirm apoptosis
- Detect caspase activity with anti-PARP pAb
- Learn about the molecular mechanisms of apoptosis
- Compare assay methods based on your needs
- Read about factors that effect viability assay outcome
- Learn how to multiplex cell viability with cytotoxicity or reporter assays
- Choose an assay with the Cell Viability Assistant
- Optimize your protein yield
- Learn about the gold standard: TNT® Systems
- Use the product selector tool to choose your system
- Explore eukaryotic, mammalian and bacterial systems
- Read about non- radioactive labeling systems
- Single gene or gene array systems
- Tips for creating a ribonuclease-free environment
- Recipes for common solutions
- Total or polyA RNA isolation systems
- Animations of MAPK and PI-3K pathways
- High or low ATP concentration kinase asssays
- Fluorescent, luminescent, radioactive and colorimetric kinase assays
- Phosphorylation-specific antibodies for MAPK pathways
- pGL4: The latest generation reporter vectors
- Dual-luciferase system: for better control
- Live-cell substrates
- Tips to maximize yield and quality
- Plasmid and genomic DNA purification
- Transfection-quality miniprep DNA
- High-, low- and medium-throughput
- DNA from blood
- Automated purification at your lab bench
- HaloTag® Technology: One construct, multiple fluors
- Live-cell imaging protocols
- Rapid labeling and imaging
- FACS®
- Affinity tags for purification
- Analyzing DNA:Protein interaction
- HaloCHIP™ System
- Pulldown assays for protein interactions
- Recipes for commonly used solutions
- Methods for achieving transfection
- Optimizing transfection efficiency
- Sample transfection protocol
- Recipes for commonly used solutions
- Learn about T-Vector cloning
- Use Flexi® Vectors for easy sequence transfer from one vector to another
- Get technical information on restriction enzymes
- Find out what affects PCR cloning
- Recipes for solutions used in cloning
- Learn about how repetitive DNA is used for human identification applications such as forensic casework and relationship testing
- Explore the steps required to generate a DNA profile
- Gain information about automated key steps in the DNA-typing process
- Discover links to Promega protocols, Profiles in DNA articles, validation guides and other resources
What's New
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Protein Expression Chapter Update
Read about new protein expression products, watch new animations and try our product selector.
