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PCR Applications

PCR Introduction
Considerations for PCR Optimization
Considerations for RT-PCR
Thermostable DNA Polymerases

Reverse Transcriptases
Example of a PCR Protocol
Example of an RT-PCR Protocol
Troubleshooting PCR and RT-PCR

RNA Interference

Introduction to RNA Interference
siRNA Design and Optimization
Enzymatic Synthesis of RNA in Vitro

DNA-Directed RNAi
RNA Delivery Strategies
Quantitating siRNA Target Gene Expression

Apoptosis

Apoptosis Introduction
Detecting Caspase Activity and Activation
Detecting Cell Death Using Mitochondrial Markers
Detecting Apoptosis by Measuring Changes in the Cell Membrane

Using DNA Fragmentation to Detect Apoptosis
Using Two or More Detection Methods to Confirm Apoptosis
Multiplexing Assays
General Protocols for Inducing Apoptosis in Cells

Cell Viability

Cell Viability Introduction
Cell Viability Assays that Measure ATP
Cell Viability Assays that Measure Metabolic Capacity

Cytotoxicity Assays That Measure the Release of LDH from Cells
Assays to Detect Apoptosis
Multiplexing Cell Viability Assays

Protein Expression

Eukaryotic In Vitro Translation Systems
Coupled Transcription/ Translation Systems
PCR DNA—Coupled Transcription/ Translation
Wheat Germ Extract Systems

Rabbit Reticulocyte Lysate Systems
Co-Translational Processing Using Canine Pancreatic Microsomal Membranes
Prokaryotic in vitro Translation Systems
Non-Radioactive Translation Detection

Expression Analysis

Expression Analysis Introduction
Techniques to Monitor Gene Expression Levels
Expression Analysis Protocols
Related Protocols

Cell Signaling

Cell Signaling Introduction
Kinase Activity Assays
Radioactive Kinase Assays

Phosphorylation-Specific Antibodies
Kinase Inhibitors
Phosphatase Assays

Bioluminescence Reporters

Bioluminescence Reporters Introduction
Luciferase Genes and Vectors
Luciferase Reporter Assays and Protocols
References

DNA Purification

DNA Purification Introduction
General Considerations for Plasmid DNA Purification
Plasmid DNA Purification Systems
Genomic DNA Isolation Systems

Specialized Genomic DNA Purification Protocol Using the MagneSil^® Genomic, Large Volume System
Fragment/PCR Product Purification Systems

Cell Imaging

Cell Imaging Introduction
HaloTag™ Interchangeable Labeling Technology
Monster Green^® Fluorescent Protein phMGFP Vector

Antibodies and Other Cellular Markers
Fluorescent Dyes

Protein Purification and Analysis

Affinity Tags
Purification of Polyhistidine-Tagged Proteins
Purification of GST-Tagged Proteins
Purification of Biotinylated Proteins

Protein:Protein Interaction Analysis: In Vivo and In Vitro Methods
Analysis of DNA-Protein Interactions
Proteomics Approaches for the Analysis of Complex Mixtures of Proteins
SDS-PAGE Analysis

Transfection

General Considerations
Factors Influencing Transfection Efficiency
Optimization of Transfection Efficiency

Promega Transfection Products
General Transfection Protocol
Stable Transfection

Cloning

Cloning Introduction
Promega Products for Cloning
PCR Cloning Protocols
Cloning With the Flexi^® Vector Systems