HaloTag^® Technology

The HaloTag^® Interchangeable Labeling Technology provides new options for rapid, site-specific labeling of proteins in living cells and in vitro. The technology is composed of HaloTag protein which is fused to your protein of interest and synthetic HaloTag ligands. The formation of a covalent bond between the HaloTag protein and synthetic ligands can impart a variety of functionalities, including fluorescence, affinity tags and direct attachment to a solid surface. For more detailed information about HaloTag, please view the online catalog page.

Click here to view an animation of how HaloTag Technology works.

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Note: For access to 20,000 human and 15,000 mouse ORF clones with HaloTag^® in multiple mammalian expression vectors and custom cloning options contact Genecopoeia at: www.genecopoeia.com/product/halo/

Imaging Applications

HaloTag^® fusion proteins can be imaged in vivo using a variety of fluorophores, allowing one DNA construct to serve as any color fluorescent protein.

• Image live cells for localization and translocation studies
• Pulse and chase with different colors for temporal studies
• Fix labeled cells and multiplex with immunocytochemisty

Live cells expressing HaloTag Protein labeled with three different ligands. HeLa cells transiently transfected with HaloTag pHT2 Vector were labeled with 5μM HaloTag TMR Ligand (Panel A); 10μM HaloTag diAcFAM Ligand (Panel B); or 25μM blue ligand; HaloTag Coumarin Ligand (Panel C).

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Protein Analysis Applications

HaloTag^® fusion proteins can be used for various types of protein analysis.

• Load labeled proteins from in vitro or in vivo expression directly on SDS-PAGE gel
• Immobilize protein complexes from cells
• Study protein:protein interaction in vitro

Efficient capture of a p65-HaloTag fusion protein and co-capture of lκB on paramagnetic streptavidin-coated particles.

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What’s your application?

Promega is constantly working on new tools and applications. To obtain the latest information on the HaloTag Technology, click here.

We are always interested in providing the solution you need. If your application could benefit from the HaloTag Technology but our current offering does not fulfill your needs, please contact us with details of your application.