FAQspeak
Is there a method for non-radioactive labeling of proteins?
The Transcend™ Non-Radioactive Translation Detection Systems (Cat.# L5070 and L5080) and the FluoroTect™ GreenLys in vitro Translation Labeling System (Cat.# L5001) can be used with any of the TNT® Coupled or Quick Coupled Transcription/Translation Systems. These systems use a precharged lysine tRNA, which is incorporated into the synthesized protein. The Transcend™ System incorporates a biotinylated lysine, which can be detected by blotting and probing with streptavidin-alkaline phosphatase or streptavidin-horseradish peroxidase, followed either by colorimetric or chemiluminescent detection. The FluoroTect™ Reagent incorporates a fluorescently labeled lysine (BODIPY®), which can be detected directly in the gel using a laser-based fluorescent gel scanner.
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