FAQspeak

How can I optimize my transfection experiments for best results?

You will need to optimize specific transfection conditions to gain optimal transfection efficiencies. The important parameters to optimize in order to maximize transfection efficiencies are the charge ratio of cationic lipid transfection reagent to DNA, the amount of transfected nucleic acid, the length of time the cells are exposed to the transfection reagent and the presence or absence of serum. Reporter genes are useful for determining optimal conditions. The transfection efficiency achieved using any transfection reagents varies depending on the cell type being transfected and the transfection conditions used. For more details on optimizing your transfection please see the transfection chapter of Promega's Protocols and Applications Guide.