FAQspeak

What factors influence the success of a transfection experiment?

With any transfection reagent or method, cell health, degree of confluency, number of passages, contamination, and DNA quality and quantity are important parameters that can greatly influence transfection efficiency. Note that with any transfection reagent or method used, some cell death will occur. Details of how these factors can affect transfection experiments are provided in the transfection chapter of Promega's Protocols and Applications Guide.

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