FAQspeak
What features of a DNA template are necessary for efficient transcription and translation in an in vitro transcription/translation system?
The most influential factors for a successful TNT® reaction are related to the DNA template and include the context of the ATG translational initiation codon, sequence and structure of the 5´ untranslated region, the presence of a synthetic poly(A) tail, form (i.e., linear v. circular), amount of DNA template and template purity. For additional information, please visit the eNotes article, Optimize Your TNT® Reticulocyte Lysate Systems.
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