What are the differences between the Wizard^® Plus Minipreps (Cat.# A7100) and the Wizard^® Plus SV Minipreps (Cat.# A1340) Systems?

The Wizard^® Plus Minipreps^(a) and Wizard^® Plus SV Minipreps^(b) DNA Purification Systems differ in their formats and in the average expected yields under standard conditions. The Wizard^® Plus Minipreps System uses a resin slurry to bind DNA. The slurry is mixed with a cleared bacterial alkaline lysate and pulled or pushed into a Wizard^® Minicolumn (Cat.# A7211) by a vacuum or syringe. In contrast, the Wizard^® Plus SV Minipreps System contains a DNA-binding membrane affixed into the Miniprep Spin Columns. As a cleared bacterial alkaline lysate is passed through the columns, DNA binding occurs. The Wizard^® Plus SV Minipreps System offers a choice of formats, spin or vacuum (SV), for purification of plasmid DNA.

For the Wizard^® Plus Minipreps System, the plasmid DNA yield from a high-copy plasmid (e.g., a pGEM^® Vector^(c)) from 1-3ml of LB medium using a standard bacterial host (i.e., JM109) is 1-10µg. For the Wizard^® Plus SV Minipreps System, up to 5ml of medium are suggested for these conditions, and yields of up to 20µg of plasmid DNA are possible. For low-copy plasmids, a larger volume of medium may be processed using the Wizard^® Plus SV Miniprep System. We do not recommend the use of a rich medium (e.g., Terrific Broth or CIRCLEGROW^® [BIO 101, Inc.]) with either system as high amounts of biomass may exceed the lytic capacity as well as lead to clogging of the columns. Both systems produce high-quality DNA suitable for many applications including restriction digests, PCR^(d), in vitro transcription/translation and fluorescent DNA sequencing.

A unique feature of the Wizard^® Plus SV Minipreps System is the use of an alkaline protease during the purification process. This protease, added to the alkaline lysate, degrades proteins to facilitate their removal from the preparation. Use of the alkaline protease in the Wizard^® Plus SV Minipreps procedure greatly reduces the likelihood of DNA degradation mediated by endonuclease A, a common endonuclease found in endA⁺ bacterial strains such as HB101 and JM101.

^(a)U.S. Pat. No. 5,658,548 and other patents.
^(b)Patent Pending.
^(c)U.S. Pat. No. 4,766,072.
^(d)The PCR process is covered by patents issued and applicable in certain countries. Promega does not encourage or support the unauthorized or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license.

pGEM and Wizard are trademarks of Promega Corporation and are registered with the U.S. Patent and Trademark Office.

CIRCLEGROW is a registered trademark of BIO 101, Inc.