Arg-C Ultra, Mass Spec Grade Technical Manual
Instructions for Use of Product(s)
VA1831, VA1832
Literature # TM754
Arg-C Ultra, Mass Spec Grade, is an endoprotease that preferentially cleaves proteins on the C-terminal side of arginine residues. The enzyme is a His-tagged cysteine protease, isolated and purified from Porphyromonas gingivalis, and is also known as gingipain R2 or RgpB. It digests proteins under reducing conditions to produce peptides that are highly suitable for identification and characterization by mass spectrometry.
Arg-C Ultra, Mass Spec Grade is highly selective for cleavage only after arginine residues, in contrast to “traditional” Arg-C (also known as clostripain), which cleaves extensively at lysine as well as arginine residues.
Summary of Changes
The following changes were made to the 5/25 revision of this document:
1. Updated attribution for two third party trademarks.
2. Corrected a typographical error.
Revised 5/25.