RiboMAX™ AOF RNA Production System
Supports Optimization of IVT Reactions Specific to Your DNA Template
- Scalable reaction conditions that reliably produce high-quality RNA
- Co-transcriptionally incorporates standard or modified nucleotides and cap analogs
- Animal-origin-free reagents suitable for RNA synthesis from research to production scale
Catalog Number:
Size
Animal Origin-Free In Vitro Transcription for RNA Therapeutics
In vitro synthesis of RNA suitable for RNA therapeutic development and production requires reactions that are not only efficient and scalable but can be adapted to maximize yield and purity for each RNA target. Maximizing reaction performance while minimizing variability, from research and early development through commercial production, is critical to success.
The RiboMAX™ AOF RNA Production System is designed to meet these demands. It features animal-origin-free (AOF), cGMP-manufactured components for robust, scalable in vitro transcription (IVT) of RNA. The system includes a standard IVT protocol along with reaction optimization guidance, which gives you the flexibility to tailor reaction conditions to your RNA target, both maximizing yield and minimizing impurities (such as double-stranded RNA).
Same Manufacturing Quality,
Additional Testing If and When You Need It
Animal Origin-Free Production – Animal origin-free manufacturing processes and raw materials.
Contamination Testing – Reduces the risks associated with contaminants that can potentially impact yield and quality of in vitro transcribed product.
Quality Documentation – Certificate of Analysis, Certificate of Origin and TSE/BSE Statements available upon request.
cGMP Reagents for mRNA Manufacturing
Trusted Technology, Modern Modalities
For over 40 years, we have been a leading provider of in vitro transcription reagents supporting a variety of applications.
To better serve and support the needs of our customers in the rapidly growing arena of mRNA therapeutic development, we have upgraded the manufacturing and quality attributes of our trusted raw material reagents to ensure they meet the strict quality requirements for the manufacture of mRNA therapeutics.
Primary Manufacturer
As a primary manufacturer, we control all aspects of product manufacturing, from raw materials through finished product. With integrated logistics support and multiple global inventory locations, we effectively eliminate uncertainty to ensure consistent supply.
Consistent Yield Efficiency and Scalable RNA Production
RNA yield from RiboMAX™ IVT reactions is scalable up to at least 10ml. Yield per milliliter of IVT reactions (left) and total yield (right) are shown. Each replicate RiboMAX™ reaction is represented as an individual bar. RiboMAX™ IVT reactions were prepared according to the protocol. Reactions (100μl, 1ml, 5ml and 10ml) were prepared in duplicate with the T7 Linear Control DNA (Part# P141A) as template (100μg/ml of reaction). No-RNA Polymerase controls (100μl) also were prepared (0.1ml – NE). Transcription reactions were incubated at 37°C for 4 hours. After transcription, RiboMAX™ reactions were diluted 100X in Nuclease-Free Water and assayed using the Qubit™ RNA BR Assay (Invitrogen) with 10μl of sample.
Comparison of Large-Scale In Vitro Transcription Kits
Comparison of large-scale IVT kits. The RiboMAX™ Large Scale RNA Production System-T7, HiScribe™ T7 High Yield RNA Synthesis Kit and TranscriptAid™ T7 High Yield Transcription Kit were used to synthesize 1.8kb luciferase transcripts and 5kb run-off transcripts containing standard nucleotides (Std.), N1-methylpseudo-UTP (U) and N1-methylpseudo-UTP + 5-methyl-CTP (U+C). Transcripts were co-transcriptionally capped using CleanCap® Reagent AG (3’ OMe) according to the protocol. Left: Transcription products were diluted 1:100 in Nuclease-Free Water and analyzed using the RNA ScreenTape Assay. The resulting gel images are displayed for transcripts synthesized using each kit. Bands are scaled to the highest peak in each lane (scaled to sample). Right: Transcripts (1.8kb) were purified using a column-based RNA cleanup kit and quantified using the QuantiFluor® RNA System (Cat.# E3310). Resulting purified 1.8kb transcript concentrations and yields are displayed.
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
rATP |
B225A | 1 × 525μl | 100mM | |
rGTP |
B226A | 1 × 525μl | 100mM | |
rCTP |
B227A | 1 × 525μl | 100mM | |
rUTP |
B228A | 1 × 525μl | 100mM | |
Magnesium Acetate |
L123A | 1 × 1000μl | 1M | |
Magnesium Chloride |
L124A | 1 × 1000μl | 1M | |
|
DTT |
V125A | 1 × 1.125ml | 1M | |
Inorganic Pyrophosphatase, AOF |
M934A | 1 × 55μl | 2u/μl | |
RNasin® Plus Ribonuclease Inhibitor, AOF |
N373A | 1 × 250μl | 40u/μl | |
T7 RNA Polymerase, AOF |
P617A | 1 × 275μl | 400u/μl | |
T7 Linear Control DNA |
P141B | 1 × 50μl | 1mg/ml | |
10X Transcription Buffer (Magnesium-Free) |
P211A | 1 × 1000μl | ||
|
Nuclease-Free Water |
P119A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Otros
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Rabbit Reticulocyte Lysate, Nuclease-Treated
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