Bio-Glo-NB™ Lytic Luciferase Assay Systems
Bright, Long-Lasting Luciferase Reagent for ADCP-MoA Target Cell Killing Assays
- Detection reagent designed for HiBiT target cell killing bioassays using primary macrophage
- Simple add-mix-read protocol for rapid assay results
- Quality controlled and tested for optimal reliability
Lytic Luciferase Assay Systems
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| JB1101 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10ml | List Price: $ 260.00 Your price: Acceder | Add to Cart | ||
| JB1102 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 100ml | List Price: $ 1,540.00 Your price: Acceder | Add to Cart | ||
| JB1103 | Bio-Glo-NB™ Lytic Luciferase Assay System | View Specifications | 10 × 100ml | List Price: $ 9,260.00 Your price: Acceder | Add to Cart |
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Quantify Targeted Cell Killing with Confidence
The Bio-Glo-NB™ Luciferase Assay Systems provide a highly sensitive, robust and homogeneous reagent for the detection of cell killing in Promega TCK Bioassays. Bio-Glo-NB™ Assay Reagents contain a cell-impermeable LgBiT that complements a HiBiT-tagged protein of interest in the extracellular media, resulting in a bright, luminescent NanoBiT® luciferase. Bio-Glo-NB™ Luciferase Assay Systems are functionally tested for performance in the Target Cell Killing Bioassays and intended for use with those products.
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Bio-Glo-NB™ Lytic Luciferase System: Assay Principle
The Bio-Glo-NB™ Lytic Luciferase Assay Systems are functionally tested for performance using ADCP-qualified macrophage in the Target Cell Killing Bioassays and intended for use with those products. When HiBiT expressing target cells are paired with primary macrophage and a biologic molecule, such as Rituximab, the target cells undergo antibody-dependent cellular phagocytosis (ADCP), which leads to degradation of HiBiT and decreased luciferase activity when LgBiT and the substrate are added.
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Simple Add-Mix-Read Protocol
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102A | 1 × 0.1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110A | 1 × 0.2ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111A | 1 × 10ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 1 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110B | 2 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 1 × 100ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ TCK LgBiT Protein |
JB102B | 10 × 1ml |
Bio-Glo-NB™ Lytic Luciferase Assay Substrate |
JB110C | 5 × 4ml |
Bio-Glo-NB™ Lytic Luciferase Assay Buffer |
JB111B | 10 × 100ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Featured Resource
The small size and bright luminescence of NanoLuc® luciferase bring exquisite sensitivity to many applications. From NanoBRET® technology, a BRET-based method to study protein:protein and protein:small molecule interactions, to NanoLuc® Binary Technology (NanoBiT), NanoLuc is the cornerstone for studying genetic responses and protein dynamics in cells at physiologically relevant levels. The flexibility of NanoLuc® technologies provides you with the bioluminescent building blocks to understand biological interactions or perform compound screening in ways that have never been possible before.
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