Filovirus HiBiT-PsVLP Bioassays
Measure Potency of Biologics and Small Molecules that Bind and Block Filovirus Entry into Target Cells
- Safe: No virus generation; suited for BSL-1/2 environments
- Fast: Rapid, complementation-based readout
- Simple: “Add-mix-read” protocol—no wash steps
- Convenient: Reagents provided in Thaw-and-Use format
Ebola (Zaire) GP HiBiT-PsVLP Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3368A09 | Early Access EBOV HiBiT-PsVLP Assay, Complete Kit | View Specifications | 1 each | Consultar Precio | |||
| CS3368A10 | Early Access EBOV HiBiT-PsVLP Assay, Complete Kit 5X | View Specifications | 1 each | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Sudan Ebola (Gulu) GP HiBiT-PsVLP Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3368A20 | Early Access SEBOV GP (Gulu) HiBiT-PsVLP, Complete | View Specifications | 1 each | Consultar Precio | |||
| CS3368A22 | Early Access SEBOV GP (Gulu) HiBiT-PsVLP, Complete 5X | View Specifications | 1 each | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Marburg Virus (Angola) GP HiBiT-PsVLP Bioassay
| Catalog No. | Product Name | Size | Price | Qty | |||
|---|---|---|---|---|---|---|---|
| CS3368A15 | Early Access MARV GP (Angola) HiBiT-PsVLP, Complete Kit | View Specifications | 1 each | Consultar Precio | |||
| CS3368A17 | Early Access MARV GP (Angola) HiBiT-PsVLP, Complete 5X | View Specifications | 1 each | Consultar Precio |
Early Access = This product is available under our Early Access program - Learn More
Catalog (FT) = This product is available under our Catalog (FT) program - Learn More
Measure the Ability of Antibodies to Neutralize Viral Entry
Neutralizing antibodies, whether generated through vaccination or produced as therapeutic monoclonal antibodies, are critical for stopping acute and chronic viral infections. Filoviruses like Ebola and Marburg are important targets for researchers developing vaccines, prophylactic monoclonal antibodies or small-molecule inhibitors. Researchers need to determine the neutralizing capacity of their therapeutic, but current methods like PRNT or PNA require infection-competent virus, stringent biosafety measures and cannot be readily implemented in most laboratories.
There are several HiBiT-PsVLP Bioassays designed for Filoviruses that utilize NPC1 for host cell entry. They provide a simple, easily implemented and robust system that can be used to measure the viral neutralization activity of both antibody and small-molecule inhibitors that block viral entry into target cells. The assay consists of a PsVLP from the Filovirus family, such as Ebola or Marburg virus, and a target cell line expressing NPC1. The PsVLP and Target Cell each contain one piece of a split luciferase technology to provide a low-background and convenient luminescent readout.
Demonstrate Filovirus Neutralization By Blocking Surface Glycoprotein Binding with NPC1 Receptor
Principle of Filovirus HiBiT-PsVLP Bioassays. 1. HiBiT-tagged VLPs pseudotyped with the virus-specific surface glycoprotein are added to the (LgBiT) Target cells. 2. In the presence of inhibitors or neutralizing antibodies (nAbs) of Filovirus entry, the entry/fusion processes of PsVLPs are blocked, thereby preventing HiBiT release, and no luminescent signal is produced. 3. In the absence of inhibitors or nAbs, HiBiT-PsVLPs bind to target cells via NPC1 and undergo membrane fusion. HiBiT is released into target cells and binds to LgBiT to generate a luminescent signal.
Assay Workflow: Standardized Reagents to Improve Your Results
- Thaw-and-use cell format provides significant time and labor savings over traditional methods while reducing variability.
- Complete Kit includes all of the required reagents in standardized formats.
- Easily amenable to high-throughput workflows.
Filoviruses: Assay Performance Data
Filovirus HiBiT-PsVLP Bioassays are suitable for blocking entry of viral particles using nAbs and small-molecule inhibitors. Virus-specific PsVLPs and HEK293T (LgBiT) Target cells were incubated with either anti-ZEBOV GP antibodies (Panel A), anti-SEBOV GP antibodies (Panel B) or anti-MARV GP antibodies (Panel C). After incubation, the Bio-Glo-NB™ VLP Reagent was added and luminescence determined in a GloMax® Discover Plate Reader.
Interested in HiBiT PsVLP Bioassays for other viruses?
Contact us to learn about our bioassay development services.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329542 | 2 × 125μl |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329543 | 2 × 2.5ml |
EBOV GP HiBiT-PsVLP |
CS3368A03 | 2 × 1.1ml |
HEK293T (LgBiT) Target Cells |
CS3368A06 | 1 × .55ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 1 × 60ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 1 × 30μl |
SDS
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NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329545 | 1 × 1.25ml |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329546 | 1 × 25ml |
HEK293T (LgBiT) Target Cells |
CS3368A06 | 5 × .55ml |
EBOV GP HiBiT-PsVLP |
CS3368A03 | 10 × 1.1ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 5 × 60ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 5 × 30μl |
SDS
Search for SDSCertificado de Análisis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
HEK293T (LgBiT) Target Cells |
CS3368A06 | 1 × .55ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 1 × 60ml |
SEBOV GP (Gulu) HiBiT-PsVLP |
CS3368A18 | 2 × 1.1ml |
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329542 | 2 × 125μl |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329543 | 2 × 2.5ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 1 × 30μl |
SDS
Search for SDSCertificado de Análisis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329545 | 1 × 1.25ml |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329546 | 1 × 25ml |
HEK293T (LgBiT) Target Cells |
CS3368A06 | 5 × .55ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 5 × 60ml |
SEBOV GP (Gulu) HiBiT-PsVLP |
CS3368A18 | 10 × 1.1ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 5 × 30μl |
SDS
Search for SDSCertificado de Análisis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
HEK293T (LgBiT) Target Cells |
CS3368A06 | 1 × .55ml |
MARV GP (Angola) HiBiT-PsVLP |
CS3368A13 | 2 × 1.1ml |
Fetal Bovine Serum |
J121A | 1 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 1 × 60ml |
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329542 | 2 × 125μl |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329543 | 2 × 2.5ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 1 × 30μl |
SDS
Search for SDSCertificado de Análisis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
Contenido
| Item | Part # | Presentación |
|---|---|---|
Bio-Glo-NB™ VLP Luciferase Substrate |
CS329545 | 1 × 1.25ml |
Bio-Glo-NB™ VLP Luciferase Assay Buffer |
CS329546 | 1 × 25ml |
HEK293T (LgBiT) Target Cells |
CS3368A06 | 5 × .55ml |
MARV GP (Angola) HiBiT-PsVLP |
CS3368A13 | 10 × 1.1ml |
Fetal Bovine Serum |
J121A | 5 × 4ml |
DMEM with 4.5g/L Glucose, L-Glutamine, Sodium Pyruvate |
J236A | 5 × 60ml |
Bio-Glo-NB™ DrkBiT Peptide (1000X) |
CS329549 | 5 × 30μl |
SDS
Search for SDSCertificado de Análisis
NOT FOR MEDICAL DIAGNOSTIC USE. FOR IN VITRO USE ONLY. BY USE OF THIS MATERIAL, RECIPIENT AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE.
This material may not be further sold or transferred by the recipient and may be used only by the recipient, and then only for research use, which includes, but is not limited to drug discovery and development, quality assurance testing, and product release assays. No other commercial use is allowed. “Commercial use” means any and all uses of this material by recipient for monetary or other consideration, including providing a service, information or data to unaffiliated third parties, and resale of this material for any use. Recipient has no right to propagate, modify, derivatize, genetically engineer or otherwise create variations of the cells or nucleotide sequence encoding the LgBiT and/or HiBiT peptides which sequences have been genetically engineered in this material. In addition, recipient must use Bio-Glo-NB™ VLP Luciferase Assay System purchased from Promega for all luminescence assays using this material or contact Promega to obtain a license for use of this material with reagents other than Promega’s.
PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THIS MATERIAL. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA.
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