Wizard® Plus SV Minipreps Start-Up Kit
Plasmid Miniprep System, Vacuum Manifold and Free Vacuum Pump
- 1–20μg high-quality plasmid DNA from 1–10ml cultures
- Process up to 20 minipreps in 45 minutes
- Choice of spin or vacuum purification formats
- For additional voltage options, contact Technical Service
Catalog Number:
Choose voltage required
Catalog Number: A6760
The Wizard® Plus SV Minipreps DNA Purification System, a silica membrane-based system, provides a simple and reliable method for rapid isolation of plasmid DNA. The entire miniprep procedure can be completed in 45 minutes or less, depending on the number of samples processed. Using the system, plasmid DNA can be purified from 1–10ml of overnight E. coli culture. The purified plasmid DNA meets a target of >98% accuracy over 500 bases using pGEM®-3Zf(+) Vector and can be used directly for automated fluorescent BigDye® terminator DNA sequencing as well as for other standard molecular biology techniques without further manipulation. It also can be used for in vitro transcription reactions when supplemented with a ribonuclease inhibitor such as Recombinant RNasin® Ribonuclease Inhibitor (Cat.# N2511).
Wizard® Plus SV Minipreps DNA Purification System allows a choice of centrifugation (spin) or vacuum-based methods for plasmid purification. Vacuum Adapters allow the use of a vacuum manifold and vacuum source for DNA purification.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
|
Vacuum Adapters |
A1331 | 1 × 20 each | |
|
Wizard® Plus SV Minipreps DNA Purification System |
A1460 | 3 × 250 preps | |
|
Welch Vacuum Pump for North America Electrical |
A6720 | 1 × 1 each | |
|
Vac-Man® Laboratory Vacuum Manifold, 20-sample capacity |
A7231 | 1 × 1 each | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Related Products
Productos Similares
Wizard® SV 96 and SV 9600 Plasmid DNA Purification Systems
Métodos de purificación de plásmidos manuales o de alto rendimiento en formato de 96 pocillos.
A2250, A2255, A2258
PureYield™ Plasmid Miniprep System
Hasta 15μg de plásmido a partir de cultivos de 3ml, listo para la transfección en 10 minutos.
A1223, A1222
PureYield™ Plasmid Midiprep System
Hasta 200µg de plásmido a partir de cultivos de 50 ml, listo para la transfección en 30 minutos.
A2492, A2495, A2496
Wizard® SV Gel and PCR Clean-Up System
Purifica fragmentos de ADN a partir de secciones de gel o de reacciones PCR, en tan sólo 15 minutos.
A9281, A9282, A9285, A9283, A9284
Usado con frecuencia junto con
pGEM®-T Vector Systems
Vector T para la clonación simplificada de productos PCR.
A3600, A3610
FuGENE® 6 Transfection Reagent
Rendimiento probado y robusto. La mejor opción para un uso diario con líneas celulares habituales.
E2691, E2692, E2693
JM109 Competent Cells
Cepa de E. coli lista para la transformación; permite el cribado azul/blanco.
L2005, L1001, L2001
