Higher Quality DNA from FFPE Samples
ReliaPrep™ FFPE gDNA Miniprep System provides a convenient method for purifying genomic DNA from formalin-fixed paraffin-embedded (FFPE) tissues. Extraction of DNA from FFPE tissues is challenging because the formalin fixation process results in cross-linking between proteins and DNA, as well as between different strands of DNA. This nonoptimal preservation of genomic DNA in FFPE complicates its use in many standard downstream analysis applications.
The ReliaPrep™ FFPE gDNA Miniprep chemistry is optimized to partially reverse this cross-linking without the need for an overnight digestion. The protocol also incorporates a deparaffinization method that does not use xylene or other hazardous or volatile solvents.
The protocol is complete in 2.5 hours with minimal hands-on time.
ReliaPrep™ FFPE Miniprep Systems Help Preserve DNA Integrity
In this experiment, 10µl of gDNA purified from FFPE human liver samples was used as template in multiplex PCR to amplify fragments of the GAPDH gene. DNA fragments ranging from 100–400bp were detected.
Purified FFPE DNA is Compatible with Sensitive Assays
Here, genomic DNA was purified from 10µm human liver FFPE sections using the ReliaPrep™ FFPE gDNA Miniprep System, and STR analysis performed using the Microsatellite Instability (MSI) Analysis System v. 1.2 (Cat.# MD1641).
Simple Protocol
Protocols
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Collection Tubes |
A130C | 1 × 10/pk | ||
|
Mineral Oil |
A244A | 1 × 5ml | ||
|
ReliaPrep™ FFPE Binding Columns |
A247A | 1 × 10/pk | ||
|
Proteinase K (PK) Solution |
A505A | 1 × 250μl | View Product | |
|
RNase A Solution |
A797D | 1 × 200μl | 4mg/ml | View Product |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Lysis Buffer (LBA) |
Z101A | 2 × 1ml | ||
|
BL Buffer |
Z103A | 1 × 3.25ml | ||
|
Wash Solution |
Z104A | 1 × 3ml | ||
Blue Dye |
MC122A | 1 × 100μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración | Available Separately |
|---|---|---|---|---|
|
Collection Tubes |
A130A | 2 × 50/pk | ||
|
Mineral Oil |
A244B | 1 × 50ml | ||
|
ReliaPrep™ FFPE Binding Columns |
A247B | 2 × 50/pk | ||
|
Proteinase K (PK) Solution |
A505B | 2 × 1.1ml | View Product | |
|
RNase A Solution |
A797C | 1 × 1ml | 4mg/ml | View Product |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Lysis Buffer (LBA) |
Z101B | 1 × 30ml | ||
|
BL Buffer |
Z103B | 1 × 32.5ml | ||
|
Wash Solution |
Z104B | 1 × 30ml | ||
Blue Dye |
MC122A | 1 × 100μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
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