CCNK Expression Vector

NV2661_CCNK Expression Vector- 20ug
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Regulatory Vector for NanoBRET® Target Engagement

  • Transfection-ready DNA to express full-length CCNK regulatory protein
  • Use with select kinase-NanoLuc® fusion vectors and NanoBRET® TE Intracellular Kinase Assay

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Catalog number selected: NV2661

$ 585.00
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CCNK Expression Vector
3 × 20μg
$ 585.00
Your price: Acceder

Express Regulatory Proteins in Mammalian Cells

The CCNK Expression Vector contains the coding region of the human CCNK regulatory protein. The expression vectors are designed for use with kinase-NanoLuc® Fusion Vectors that contain the coding region of a kinase-NanoLuc® (or NanoLuc®-kinase) fusion protein. The Expression Vector and kinase-NanoLuc® Fusion Vector are cotransfected into cells prior to analysis using the appropriate NanoBRET® Target Engagement Intracellular Kinase Assay. These regulatory protein Expression Vectors are supplied transfection-ready, with a CMV promoter that drives expression of the full-length regulatory protein.

For details on kinase-NanoLuc® Fusion Vectors that require co-expression with a regulatory protein Expression Vector, see the NanoBRET® Target Engagement Intracellular Kinase Assay Adherent Format Technical Manual, #TM598.

Vector Details:

  • Gene name: CCNK
  • Sequence
  • Recommended Assay: NanoBRET® TE Intracellular Kinase Assay, Adherent Format

NanoBRET® Target Engagement (TE) Intracellular Kinase Assays

The NanoBRET® TE Intracellular Kinase Assays can analyze the affinity of test compounds by competitive displacement of a fluorescent NanoBRET® Tracer reversibly bound to a NanoLuc®-kinase fusion protein in cells. Introduction of competing test compounds results in a dose-dependent decrease in the NanoBRET® signal for estimating intracellular affinity against the target protein. The NanoBRET® Tracer used is determined by your choice of NanoLuc® kinase fusion vector. The Kinase Vector Selection table shows which NanoBRET® Tracer assay is correct for each kinase fusion vector.

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Contenido

Item Part # Presentación Concentración

CCNK Expression Vector

NV266A 3 × 20μg 1μg/μl

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

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Patentes y Exclusiones

Licensed from Kazusa Genome Technologies.

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