PKC nu Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The PKC nu Kinase Enzyme System contains:

• PKC nu Kinase, 10μg (Human, full-length recombinant). MW: ~142kDa
• CREBtide substrate (1mg/ml), (KRREILSRRPSYR); derived from human CREB1 isoform A (amino acid 109–121)
• 5X Reaction Buffer, 0.1M DTT

Recombinant full-length human PKC nu was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. PKC nu, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKC nu is composed of 890 amino acid residues and the protein has 77.3% similarity to human PKCμ and 77. 4% similarity to mouse PKD.

Application Note

NCBI Database Entry

The PKC nu Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.