MSK2 Kinase Enzyme System

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The MSK2 Kinase Enzyme System contains:

• MSK2 Kinase, 10μg (Mouse, recombinant full-length). MW: ~114kDa.
• RSK Substrate (KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acid 230–239).
• Reaction Buffer, DTT.

Recombinant full-length mouse MSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MSK2 or mitogen- and stress-activated protein kinase-2 is a member of the serine/threonine kinases family that contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and c-fos. MSK2 is also known as ribosomal protein S6 kinase 4 (RPS6KA4) that is activated by the mitogen-activated protein kinases ERK1, ERK2 and p38.

MSK2 NCBI Database Entry.

The MSK2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

See all Kinase Enzyme Systems available from Promega.