MET (M1250T) Kinase Enzyme System

Kinase Enzyme Systems for Detecting Kinase Activity
customize-this-small
View information on global supply logistics

Easily Screen and Profile MET (M1250T) Kinase Inhibitors

  • Includes kinase, substrate and reaction buffer
  • Use with ADP-Glo™ Assay for bioluminescent detection of kinase activity

Size

Additional options

Catalog number selected: V4168

$ 545.00
Your price:
Add to Cart
This product is discontinued
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
MET (M1250T) Kinase Enzyme System
10µg
$ 545.00
Your price: Acceder
Cambiar Configuración

Convenient, Scalable Kinase Profiling

The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The MET (M1250T) Kinase Enzyme System contains:

  • MET (M1250T) Kinase, 10μg (Human, recombinant; amino acids 956–end). MW: ~81kDa.
  • Poly (4:1 Glu, Tyr) Peptide Substrate.
  • Reaction Buffer, DTT, MnCl2.

Recombinant human MET (point mutant M1250T; amino acids 956–end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MET is a proto-oncogene that encodes a transmembrane growth factor receptor, which is a heterodimer of two disulphide-linked chains of 50kDa (α) and 145kDa (β). MET is widely expressed in the kidney, brain, lung, skin and embryonic tissue. Hepatocyte growth factor (HGF) binds to MET and activates its tyrosine kinase activity. MET is overexpressed and activated in a variety of human cancers including pancreatic, colon, gastric, cervical and ovarian and has been shown to be involved in tumor cell migration and invasion.

MET (M1250T) NCBI Database Entry.

The MET (M1250T) Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.

Use with ADP-Glo™ Kinase Assay

The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.

 

See all Kinase Enzyme Systems available from Promega.

Especificaciones

You are viewing: V4168 Cambiar Configuración

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

AA

Especificaciones

You are viewing: V6448 Cambiar Configuración

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

AA

Especificaciones

You are viewing: V4169 Cambiar Configuración

Contenido

Item Part # Presentación
MET (M1250T) Kinase Enzyme System V4168 1 × 10μg

ADP-Glo™ Kinase Assay

V9101 1 × 1,000 assays

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Encontremos el producto que se adapte a sus necesidades.

Hablar con un científico

Manas

Manas

India