Lumit® hKi-67 Immunoassay for Cell Proliferation
No-Wash Assay for Ki-67 Detection
- Increase confidence in your data by tracking a well-known marker of cell proliferation
- Decrease prep time using a fast, no-wash, add-and-read protocol
- Make quicker decisions by using an earlier readout of proliferation
- Available through our Early Access program
Catalog Number:
Size
Catalog Number: CS3076A01
Assess Cell Proliferation in Less Than Two Hours with “Add-Mix-Measure” Assay
Cell proliferation is a fundamental parameter of cell-based research. Investigators currently monitor cell proliferation with methods that can be affected by changes in metabolism, require cumbersome wash steps or use indirect markers of proliferation, radioactive material and specialized equipment.
The Lumit® hKi-67 Immunoassay for Cell Proliferation is an easy add-mix-measure plate-based assay for tracking Ki-67, a well-known marker of cell proliferation, without washing steps or specialized detection equipment. A reader capable of detecting luminescence is the only instrument required.
With this assay, researchers can generate more data with higher confidence and reduce prep work and time to results.
How the Lumit® hKi-67 Immunoassay Works
Primary antibodies to Ki-67 were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of Ki-67, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to Ki-67 levels.
Avoid Tedious Wash Steps with Simple Protocol
Broad Linear Range and Specificity of Lumit® hKi-67 Detection
The broad linear detection range of the Lumit® hKi-67 Immunoassay covers expected Ki-67 levels for most cell models and is specific to Ki-67 detection.
Measure Decreases in Cell Proliferation
Treatment of HCT 116 cells (10,000/well, 96-well plate) with increasing concentrations of the antiproliferative agent nutlin-3A did not induce cytotoxicity and reduced Ki-67 expression levels in a dose-dependent manner. Ki-67 levels were assessed with the Lumit® hKi-67 Assay, while changes in viable cell number were determined with CyQUANT™ Direct in a parallel assay plate.
Measure Increases in Cell Proliferation
Human CD8+ T cells (StemCell Tech) plated at 80,000 cells per well were treated with CD3/CD28 T cell activator with (orange) and without (blue) 10ng/ml IL-2 for 48 hours. Upregulation of Ki-67 is observed with the Lumit® hKi-67 Immunoassay before T cell proliferation (which begins >72 hours after activation), demonstrating use of this assay as an early indicator of proliferation.
Need to differentiate between antiproliferative effects and cell death?
Quickly and easily assess cell proliferation with the Lumit® hKi-67 Immunoassay for Cell Proliferation, a homogeneous cell-based assay for the detection of hKi-67, a key marker of cell proliferation.
Blog Article: Rethinking Cell Proliferation Assays
The innovative Lumit® hKi-67 Immunoassay for Cell Proliferation tracks a marker that is independent of the metabolic state of cells, enabling researchers to be more confident in their assessment of proliferation.
Protocols
No protocols available
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación |
|---|---|---|
Lumit® Detection Substrate C |
CS3076A02 | 1 × 480μl |
Lumit® Immunoassay Buffer C (10X) |
CS3076A03 | 1 × 1.8ml |
Lumit® Lysis Buffer II (10X) |
CS3076A04 | 1 × 1.3ml |
Human Ki-67 Protein, Partial |
CS3076A05 | 1 × 25μl |
Anti-hKi-67 mAb-LgBiT, 400X |
CS3076A06 | 1 × 60μl |
Anti-hKi-67 mAb-SmBiT, 400X |
CS3076A07 | 1 × 60μl |
CellTox™ Green Dye, 1,000X |
G873A | 1 × 20μl |
U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.
U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 11,493,504; European Pat. No. 2970412; Japanese Pat. Nos. 7280842 and 7532562; and other patents and patents pending.
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