Luciferase genes from the North American firefly (Photinus pyralis) and from other beetles are commonly used as reporter genes for studying transcription regulation in transient assay systems and as markers for stably transformed eukaryotic cells. Beetle luciferin (also known as d-luciferin) is synthesized as the monopotassium salt and is a substrate for the beetle luciferase reporter systems. d-luciferin is provided for those researchers who prefer to formulate their own assay reagents for monitoring in vitro or in vivo luciferase activity.
Formula: C11H7N2O3S2•K.
Formula Weight: 318.4 (anhydrous).
Formulation: Supplied as a potassium salt for easy preparation in aqueous buffer.
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Reference
- Ow, D.W. et al. (1986) Science 234, 856.