Using Real-Time Assays to Monitor Cell Health In Vitro: A PBMC Case Study
In this webinar, you will learn:
- How to multiplex viability and cytotoxicity assays and monitor these conditions in real-time
- To what extent PBMC cell density affects cellular health assays
- If in vivo cell culture for up to 72 hours influences the health of isolated PBMCs
Summary
Are you interested in assessing the function or viability of PBMCs in vitro? Do you want to know if extended culture influences PBMC viability and/or cytotoxicity? Peripheral blood mononuclear cells (PBMCs) are one of the most widely used models in biomedical research. However, the stability of PBMCs in culture post isolation is not well documented. Typically, PBMC viability or cytotoxicity is measured at a single time point after isolation, providing a limited snapshot of PBMC health for assays that last over multiple days. Through the implementation of multiplexed cellular health assays, a more comprehensive analysis becomes feasible, enabling the observation of real-time effects of in vitro culture on PBMC viability and cytotoxicity. Join our featured speakers to learn how multiplexing real-time cellular health assays can inform on changes in PMBC viability and cytotoxicity over extended in vitro culture.
Speakers
Kyle Hooper, PhD
Segment Marketing Manager
Promega Corporation
Kyle came to Promega in 1996 after a post-doc at the University of Texas Southwestern Medical Center and PhD degree from the University of Kansas Medical Center. Kyle has served roles in technical support, technical training, product development and global marketing support specializing in bioluminescent technologies.
Jonathan Barlow, PhD
Scientific Research Director
Cellular Health and Metabolism Facility
University of Birmingham, UK
Dr. Barlow has more than 10 years’ experience using extracellular flux analysis and fluorescent based cellular health assays for assessing cellular health and metabolic function in cells and tissues. His expertise covers in-depth knowledge of cellular bioenergetics and cell health assays with specific skills using Seahorse Extracellular Flux (XF) Analysers and multimode optical plate readers. His own research interests are targeted at enhancing and improving methodological approaches to explore cellular health and metabolism in vitro. Dr. Barlow delivers training workshops at the cellular health and metabolism facility in Birmingham on methods used to explore ‘real-time’ cellular health and metabolic function in vitro. He offers academic consultancy to companies and institutions that require bespoke metabolic assay development using Seahorse XF technology and/or require assistance with analysing and interpreting Seahorse XF analysis data.