Advances in Glucagon Detection Supporting Diabetes and Metabolic Disorder Research

This webinar is Part 4 of the Lumit™ Technology Webinar Series.

In this webinar, you will learn:

  • About the importance of glucagon in blood glucose homeostasis and how its regulation goes awry during diabetes
  • How better understanding of alpha cell signaling can inform new therapeutic strategies for treating diabetes
  • How detection with Lumit™ speeds up analysis of these samples, allowing you to get results faster

Summary

Lumit™ Technology Webinar Series

Immunoassays are an essential tool for life science researchers, widely used for decades. Applying bioluminescent technology to this well-accepted assay format to create Lumit™ Immunoassays has made analyte detection faster and easier than ever before. This webinar series will introduce you to Lumit™ Immunoassay technology and highlight key research areas where bioluminescent immunoassays have supported research discoveries.

Part 4: Advances in Glucagon Detection Supporting Diabetes and Metabolic Disorder Research

In the fourth part of this series, Dr. Donna Leippe, Promega, will introduce  Lumit™ Glucagon and Insulin Immunoassays and Dr. David Piston, Washington University St Louis, will describe how his lab uses islet perifusion as a key tool in their diabetes research. This model generates hundreds of samples per experiment and requires high-throughput immunoassays to measure insulin and glucagon secreted from the islets. See how Lumit™ Immunoassay technology speeds up the analysis of these samples, allowing you to get results faster. 

Other Webinars in this Series:

Bioluminescent Analyte Detection with Lumit™ Immunoassays

Using Lumit™ Technology to Address Inflammasome-mediated Cytokine Release

Ask the Experts Forum: Implementing Lumit™ Immunoassays

Optimizing IL-6 Detection for High-Throughput Screening


Speakers

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Donna Leippe, PhD
Senior Research Scientist
Promega

Dr. Donna Leippe has spent over 18 years developing technologies and leading research projects as a Senior Research Scientist in R&D at Promega. She is a member of the Assay Design group and focuses on developing bioluminescent technologies for cellular metabolism research.  This has included assays for measuring cellular cofactors (NAD/NADH-Glo™ and NADP/NADPH-Glo™ Assays) and multiple cellular metabolites (Lactate-Glo™, Glucose-Glo™, Glutamate-Glo™ and Glutamine/Glutamate-Glo™ Assays). Donna joined Promega after completing her graduate and postdoctoral work at the University of Wisconsin-Madison, where she studied Viral Immunology at the Institute for Molecular Virology and the McArdle Laboratory for Cancer Research.
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David Piston, PhD
Edward J. Mallinckrodt, Jr. Professor and Head of Cell Biology and Physiology
Washington University School of Medicine, St. Louis

David W. Piston is the Mallinckrodt Professor and Head of the Department of Cell Biology and Physiology at Washington University in St. Louis.  Research in the Piston lab focuses on understanding glucose-regulated hormone secretion from islets of Langerhans, which is a micro-organ consisting of ~1,000 cells.  The lab has developed live cell imaging methods to assay the glucose transduction pathway, and correlate these assays with functional data to bridge the gap between the known details from individual cells and the overall glucose response of a whole islet.  Over the last 10 years, the team has focused on the glucagon-secreting α-cells, whose function remains enigmatic.  They have characterized modulation of glucagon secretion by multiple regulatory pathways including cAMP/PKA, EphA forward signaling, and α-cell heterogeneities in electrical activity.  The Piston lab has over 30 years of successful collaborations with many other labs in diabetes and other research fields.