Technologies to Explore Kinase Biology – From Basic Research to Drug Discovery
- How kinase activity can be reliably determined in a biochemical format using Kinase Enzyme Systems & ADP-Glo®
- How to characterize intracellular drug:kinase interaction using NanoBRET® Target Engagement
- How to investigate kinase-mediated signaling using cellular assays based on the NanoBiT® technology
Summary
The ADP-Glo™ Assays in combination with the Kinase Enzyme Systems provide a convenient biochemical method for profiling the effect of chemical compounds on kinase activity. With more than 340 Kinases, the NanoBRET® Target Engagement Intracellular Kinase Assays provide an excellent tool to characterize drug:kinase interaction in live cells under physiologically relevant conditions. These assays allow to quantitatively measure test compound affinity, fractional occupancy as well as the durability of the interaction (residence time). NanoBiT® technology is another powerful tool to investigate cellular kinase biology. The NanoBiT® PPI System or the Lumit® Immunoassays enable an in-depth study of kinase-mediated signaling.
You will learn:
- How kinase activity can be reliably determined in a biochemical format using Kinase Enzyme Systems and ADP-Glo®
- How to characterize intracellular drug:kinase interaction using NanoBRET® Target Engagement
- How to investigate kinase-mediated signaling using cellular assays based on the NanoBiT® technology
Speaker
Erik Bonke, PhD
Field Application Specialist
Promega GmbH
Erik Bonke received a Diploma in biology from the Johannes-Gutenberg University in Mainz, Germany in 2012. In his diploma thesis at the University Hospital in Mainz he worked at the genetic manipulation of murine embryonic stem cells in order to generate different transgenic mouse strains. He did his doctoral thesis at the University Hospital in Frankfurt am Main, where he focused on the mechanistic fundamentals of mitochondrial ROS generation and their physiological implication as cellular second messenger molecules, a process termed redox signaling. After completion of the experimental part of his thesis, Mr. Bonke joined Promega Germany to work as an Application Specialist with a main focus on cellular reporter technologies. As part of this position, he is giving frequent practical as well as theoretical workshops/seminars on the application of Promega's current luminescent reporter portfolio. In 2018, he was awarded a doctoral degree by the Johann Wolfgang Goethe University Frankfurt.