Rapid Ligation for the pGEM®-T and pGEM®-T Easy Vector Systems

Susan Frackman and Dan Kephart
Promega Corporation
Publication Date: 1999

Abstract

The pGEM®-T and pGEM®-T Easy Systems are now provided with a new 2X Rapid Ligation Buffer that allows the user to perform ligation reactions in as little as one hour. In this study the performance of the 2X Rapid Ligation Buffer is compared with that of the previously supplied T4 DNA Ligase 10X Buffer in both one-hour and 16-hour ligation reactions. The effects of incubation time, ligation reaction temperature, and insert:vector ratio on cloning efficiencies are also evaluated using the 2X Rapid Ligation Buffer.

Promega Notes 71, 8.