PowerPlex® ESI 17 Pro System for Use on the Spectrum Compact CE System Technical Manual
Instructions for Use of Product(s)
DC7780
Literature # TMD065
The PowerPlex® ESI 17 Pro System is an STR multiplex amplification kit that is used for human identification applications including forensic analysis, relationship testing and research use. This system allows co-amplification and four-color fluorescent detection of seventeen loci (sixteen STR loci and Amelogenin), including D22S1045, D2S1338, D19S433, D3S1358, Amelogenin, D2S441, D10S1248, D1S1656, D18S51, D16S539, D12S391, D21S11, vWA, TH01, SE33, FGA and D8S1179. The PowerPlex® ESI 17 Pro System is designed with six of the original seven European Standard Set (ESS) loci (D3S1358, D18S51, TH01, vWA, D8S1179 and the more common FGA alleles) along with D16S539 and D19S433 as smaller amplicons (<250bp), while the loci recommended by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) (D1S1656, D2S441, D10S1248, D12S391 and D22S1045) are present as larger amplicons. The PowerPlex® ESI 17 Pro System provides all necessary materials to amplify STR regions of human genomic DNA, including a hot-start thermostable Taq DNA polymerase. This manual contains a protocol to separate amplified products and detect separated material on the Spectrum Compact CE System.
Summary of Changes
The following changes were made to the 9/21 revision of this document:
- Expanded character rules table in Section 5.3.
- Updated disclaimers.
Revised 9/21.